Anti-TWEAK antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (AB37170)

Immunohistochemistry analysis Formalin/PFA-fixed paraffin-embedded human brain tissue sections labeling TWEAK with ab37170 at 10 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (AB37170)

Immunohistochemistry analysis Formalin/PFA-fixed paraffin-embedded mouse brain tissue sections labeling TWEAK with ab37170 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (AB37170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining TWEAK with ab37170 at 20µg/ml

• WB

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Western blot - Anti-TWEAK antibody (AB37170)

Western Blot of HeLa cell lysate labeling TWEAK with Anti-TWEAK antibody (ab37170) at (1) 1 and (2) 2μg/ml.

All lanes:

Western blot - Anti-TWEAK antibody (ab37170)

Predicted band size: 27 kDa

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• WB

Supplier Data

Western blot - Anti-TWEAK antibody (AB37170)

Primary incubation time : 1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-TWEAK antibody (ab37170) at 2 µg/mL

All lanes:

Mouse Liver Tissue Lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 27 kDa

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• WB

Supplier Data

Western blot - Anti-TWEAK antibody (AB37170)

Primary incubation : 1h incubation at RT in 5% NFDM/TBST

Lane 1:

Western blot - Anti-TWEAK antibody (ab37170) at 1 µg/mL

Lane 2:

Western blot - Anti-TWEAK antibody (ab37170) at 2 µg/mL

All lanes:

Mouse pancreas tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 27 kDa

false

• WB

Unknown

Western blot - Anti-TWEAK antibody (AB37170)

TWEAK migrates at aberrant positions in SDS-PAGE; hence the observed band is different from the predicted molecular weight of 27 kDa.

All lanes:

Western blot - Anti-TWEAK antibody (ab37170) at 1 µg/mL

Lane 1:

Recombinant human TWEAK at 0.005 µg

Lane 2:

Recombinant human TWEAK at 0.025 µg

Lane 3:

Recombinant human TWEAK at 0.05 µg

Predicted band size: 27 kDa

Observed band size: 37 kDa

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