Rabbit Polyclonal TWEAK antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNFSF12.
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- Skeletal muscle 12:182022Dysregulation of Tweak and Fn14 in skeletal muscle of spinal muscular atrophy mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina E Meijboom,Emma R Sutton,Eve McCallion,Emily McFall,Daniel Anthony,Benjamin Edwards,Sabrina Kubinski,Ines Tapken,Ines Bünermann,Gareth Hazell,Nina Ahlskog,Peter Claus,Kay E Davies,Rashmi Kothary,Matthew J A Wood,Melissa BowermanPubMed 35902978
- Cell death discovery 8:3282022Inhibition of TWEAK/Tnfrsf12a axis protects against acute liver failure by suppressing RIPK1-dependent apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijie Li,Heming Wang,Junjin Zhu,Ning Nan,Yi Lin,Xuran Zhuang,Ling Li,Yamin Zhang,Pengyu HuangPubMed 35853848
- Molecular biology reports 47:5165-51732020Parthenolide ameliorates tweak-induced podocytes injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLichun Yu et. al.PubMed 32572732
- EBioMedicine 46:274-2892019A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNerea Méndez-Barbero et. al.PubMed 31395500
- International journal of molecular sciences 20:2018Exercise Training Impacts Cardiac Mitochondrial Proteome Remodeling in Murine Urothelial Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRita Ferreira et. al.PubMed 30602657
- Development (Cambridge, England) 145:2018TWE-PRIL reverse signalling suppresses sympathetic axon growth and tissue innervation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Howard et. al.PubMed 30337376
- Molecular medicine reports 19:41-502018Hyperoside decreases the apoptosis and autophagy rates of osteoblast MC3T3‑E1 cells by regulating TNF‑like weak inducer of apoptosis and the p38mitogen activated protein kinase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Zhang et. al.PubMed 30387825
- Archives of biochemistry and biophysics 645:12-182018Exercise training protects against cancer-induced cardiac remodeling in an animal model of urothelial carcinoma.
- Tobacco induced diseases 15:432017Tumor necrosis factor-like weak inducer of apoptosis regulates quadriceps muscle atrophy and fiber-type alteration in a rat model of chronic obstructive pulmonary disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Juan Lu et. al.PubMed 29151827
- Frontiers in cellular and infection microbiology 7:3152017Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TNFSF12. Database link O43508
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes TWEAK may migrate at aberrant poitions in SDS-PAGE. |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes TWEAK may migrate at aberrant poitions in SDS-PAGE. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-10.00000 µg/mL | Notes - |
Species Human | Dilution info 5.00000-10.00000 µg/mL | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Alternative names
APO3L, DR3LG, UNQ181/PRO207, TNFSF12, Tumor necrosis factor ligand superfamily member 12, APO3 ligand, TNF-related weak inducer of apoptosis, TWEAK
Recommended products
Rabbit Polyclonal TWEAK antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TNFSF12.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TNFSF12. Database link O43508
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TNF-like weak inducer of apoptosis (TWEAK) also known as CD255 is a cytokine involved in a variety of cellular processes. This protein has an approximate mass of 24 kDa. TWEAK is expressed in many tissues including the heart brain and spleen. It functions by binding to the fibroblast growth factor-inducible 14 (Fn14) receptor leading to the activation of downstream signaling pathways. TWEAK's role as a ligand places it in the tumor necrosis factor (TNF) superfamily highlighting its involvement in cell death and inflammation signaling.
- Biological function summary
TWEAK modulates processes like cell proliferation differentiation and apoptosis. This cytokine affects the immune response and tissue regeneration becoming significant in processes that require tissue remodeling. While acting independently TWEAK does not form part of a larger protein complex. Instead it signals mainly through its exclusive receptor Fn14 on responsive cells triggering cellular responses that influence its biological activities.
- Pathways
TWEAK plays an important role in the NF-kB and MAPK signaling pathways. These pathways are important for immune response regulation and cell survival. In these pathways TWEAK closely interacts with TNF receptor-associated factors (TRAFs) which facilitate TWEAK signaling. The connections to pathways involving inflammatory response highlight its significance in maintaining homeostasis within the cellular environment.
- Associated diseases and disorders
TWEAK's activity links to conditions like cancer and rheumatoid arthritis. In cancer excessive TWEAK signaling can contribute to tumor growth and metastasis through its pro-inflammatory and pro-angiogenic effects. In rheumatoid arthritis TWEAK expression relates to joint inflammation and tissue damage where it interacts with proteins like IL-6 and TNF-alpha. These interactions suggest potential therapeutic targets by modulating TWEAK or its signaling partners to treat these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (ab37170)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining TWEAK with ab37170 at 20µg/ml
Western blot - Anti-TWEAK antibody (ab37170)
Western Blot of HeLa cell lysate labeling TWEAK with Anti-TWEAK antibody (ab37170) at (1) 1 and (2) 2μg/ml.
All lanes: Western blot - Anti-TWEAK antibody (ab37170)
Predicted band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (ab37170)
Immunohistochemistry analysis Formalin/PFA-fixed paraffin-embedded human brain tissue sections labeling TWEAK with ab37170 at 10 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAK antibody (ab37170)
Immunohistochemistry analysis Formalin/PFA-fixed paraffin-embedded mouse brain tissue sections labeling TWEAK with ab37170 at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-TWEAK antibody (ab37170)
Primary incubation: 1h incubation at RT in 5% NFDM/TBST
Lane 1: Western blot - Anti-TWEAK antibody (ab37170) at 1 µg/mL
Lane 2: Western blot - Anti-TWEAK antibody (ab37170) at 2 µg/mL
All lanes: Mouse pancreas tissue lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 27 kDa
Western blot - Anti-TWEAK antibody (ab37170)
Primary incubation time: 1h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-TWEAK antibody (ab37170) at 2 µg/mL
All lanes: Mouse Liver Tissue Lysates at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 27 kDa
Western blot - Anti-TWEAK antibody (ab37170)
TWEAK migrates at aberrant positions in SDS-PAGE; hence the observed band is different from the predicted molecular weight of 27 kDa.
All lanes: Western blot - Anti-TWEAK antibody (ab37170) at 1 µg/mL
Lane 1: Recombinant human TWEAK at 0.005 µg
Lane 2: Recombinant human TWEAK at 0.025 µg
Lane 3: Recombinant human TWEAK at 0.05 µg
Predicted band size: 27 kDa
Observed band size: 37 kDa
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