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AB288424

Anti-TWEAK antibody [MTW-1]

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Rat Recombinant Monoclonal TWEAK antibody. Suitable for ICC/IF and reacts with Mouse samples. Immunogen corresponding to Recombinant Protein within Mouse Tnfsf12.

View Alternative Names

Tumor necrosis factor ligand superfamily member 12, TNF-related weak inducer of apoptosis, TWEAK, Tnfsf12

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-TWEAK antibody [MTW-1] (AB288424)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TWEAK antibody [MTW-1] (AB288424)

The image data was generated using the chimeric rabbit IgG version of the same antibody clone

Immunofluorescence staining of mouse splenocytes using anti-TWEAK antibody MTW-1. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of MTW-1 at 10 μg/ml followed by Alexa Fluor® 488 secondary antibody (2 μg/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom anti-TWEAK antibody, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MTW-1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Recombinant Protein within Mouse Tnfsf12. The exact immunogen used to generate this antibody is proprietary information.

O54907

Specificity

This antibody is specific for murine TWEAK. It has been shown not to react with TNF-α, FasL, and TRAIL

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Complementary Products

Assay Kits

AB267635

Human TWEAK ELISA Kit

Sandwich ELISA - Human TWEAK ELISA Kit (AB267635)

  • 0
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Primary Antibodies

AB109365

Anti-TWEAKR/FN14 antibody [EPR3179]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365)

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Assay Kits

AB267635

Human TWEAK ELISA Kit

Sandwich ELISA - Human TWEAK ELISA Kit (AB267635)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TNF-like weak inducer of apoptosis (TWEAK) also known as CD255 is a cytokine involved in a variety of cellular processes. This protein has an approximate mass of 24 kDa. TWEAK is expressed in many tissues including the heart brain and spleen. It functions by binding to the fibroblast growth factor-inducible 14 (Fn14) receptor leading to the activation of downstream signaling pathways. TWEAK's role as a ligand places it in the tumor necrosis factor (TNF) superfamily highlighting its involvement in cell death and inflammation signaling.
Biological function summary

TWEAK modulates processes like cell proliferation differentiation and apoptosis. This cytokine affects the immune response and tissue regeneration becoming significant in processes that require tissue remodeling. While acting independently TWEAK does not form part of a larger protein complex. Instead it signals mainly through its exclusive receptor Fn14 on responsive cells triggering cellular responses that influence its biological activities.

Pathways

TWEAK plays an important role in the NF-kB and MAPK signaling pathways. These pathways are important for immune response regulation and cell survival. In these pathways TWEAK closely interacts with TNF receptor-associated factors (TRAFs) which facilitate TWEAK signaling. The connections to pathways involving inflammatory response highlight its significance in maintaining homeostasis within the cellular environment.

TWEAK's activity links to conditions like cancer and rheumatoid arthritis. In cancer excessive TWEAK signaling can contribute to tumor growth and metastasis through its pro-inflammatory and pro-angiogenic effects. In rheumatoid arthritis TWEAK expression relates to joint inflammation and tissue damage where it interacts with proteins like IL-6 and TNF-alpha. These interactions suggest potential therapeutic targets by modulating TWEAK or its signaling partners to treat these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity).
See full target information Tnfsf12
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Product promise

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