Anti-TWEAKR/FN14 antibody [EPR3179]

• IP

Lab

Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365)

ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

Lane 1 (input) : HUVEC whole cell lysate 10ug

Lane 2 (+) : ab109365 + HUVEC whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Predicted band size: 14 kDa

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• WB

Unknown

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365)

All lanes:

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

Lane 1:

HT-29 cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

HT-1080 cell lysate at 10 µg

Predicted band size: 14 kDa

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• WB

Unknown

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365)

Lanes 1 - 2:

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] - Low endotoxin, Azide free (<a href='/en-us/products/primary-antibodies/tweakr-fn14-antibody-epr3179-low-endotoxin-azide-free-ab228481'>ab228481</a>) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein/vascular endothelium) whole cell lysate at 20 µg

Lane 2:

HT-1080 (human fibrosarcoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa,70 kDa

Observed band size: 110 kDa,14-17 kDa

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• WB

Unknown

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365)

All lanes:

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

Lane 2:

L6 (rat skeletal muscle) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse muscle) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa

Observed band size: 14-17 kDa

false

• WB

CiteAb

Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365)

TWEAKR/FN14 western blot using anti-TWEAKR/FN14 antibody [EPR3179] ab109365. Publication image and figure legend from Zou, Y., Lee, J., et al., 2014, PLoS One, PubMed 25222179.

ab109365 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109365 please see the product overview.

Protein expression of CD133, Fn14, TFF3, HNF4α, p-Akt1 (T308), Akt1, p-p70S6K (T389), p70S6K, p-4E-BP1 (T37/46), and 4E-BP1 in regenerating livers of Nrf2+/+ and Nrf2−/− mice.Livers were collected from normal mice and the mice subjected to partial hepatectomy (PH) at the indicated time points following surgery. Liver lysates prepared from three mice per time point per genotype were pooled with equal amount of protein from each preparation. Western blot was performed with antibodies against the proteins indicated. Glyceraldehyde 3-phosphate dehydrogenase (GADPH) was used as a loading control. NL, normal liver.

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