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Rabbit Recombinant Monoclonal TWEAKR/FN14 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 24 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365), expandable thumbnail
  • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365), expandable thumbnail
  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (AB109365), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWB
Human
Tested
Not recommended
Tested
Tested
Mouse
Expected
Not recommended
Expected
Tested
Rat
Expected
Not recommended
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/50 - 1/500

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/50 - 1/500

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50 - 1/500

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/40

Notes

For unpurified use at 1/10.

Expected
Expected

Species

Mouse

Dilution info

1/40

Notes

For unpurified use at 1/10.

Species

Rat

Dilution info

1/40

Notes

For unpurified use at 1/10.

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

1 product for Alternative Version

Target data

Function

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

Alternative names

Tumor necrosis factor receptor superfamily member 12A, Fibroblast growth factor-inducible immediate-early response protein 14, Tweak-receptor, FGF-inducible 14, TweakR, TNFRSF12A, FN14

Recommended products

Rabbit Recombinant Monoclonal TWEAKR/FN14 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 24 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3179

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The TWEAKR/FN14 known also as FN14 protein is a receptor primarily involved in cellular signaling. It is found on the cell surface and has a molecular weight of approximately 14 kDa. This receptor expresses in various tissues but its expression is especially high in the liver kidney and muscle tissues. Through its engagement with its ligand TWEAK (Tumor Necrosis Factor-Like Weak Inducer of Apoptosis) TWEAKR/FN14 participates in signal transduction processes that regulate cell survival and proliferation.

Biological function summary

TWEAKR/FN14 influences multiple cellular activities including inflammation proliferation and apoptosis. It does not solely function in isolation often interacting with other proteins in complex networks. In fact FN14 often couples its activity with downstream signaling proteins that impact pathways involved in tissue remodeling and repair. The receptor's ability to mediate these processes makes it a significant regulator of tissue response to damage.

Pathways

Signaling through TWEAKR/FN14 primarily involves the NF-κB and MAPK pathways. These signaling pathways are critical for mediating cellular responses to external stimuli such as stress or injury. The NF-κB pathway in particular plays a role in immune and inflammatory responses where FN14 through its interaction modulates these responses. Additionally proteins such as TRAF (TNF Receptor Associated Factor) are related to FN14 through these pathways contributing to its signaling functions.

Associated diseases and disorders

Dysregulation of TWEAKR/FN14 is linked to pathological conditions such as cancer and fibrosis. Overexpression of FN14 has been observed in several malignancies and its interaction with TWEAK has been implicated in tumorigenic processes. Meanwhile in fibrosis FN14 is associated with excessive tissue repair and scarring connected to the accumulation of extracellular matrix proteins. Altered expression levels and activity of FN14 with its partner proteins can contribute to the progression of these diseases highlighting its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

    Lane 1 (input): HUVEC whole cell lysate 10ug

    Lane 2 (+): ab109365 + HUVEC whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109365 in HUVEC whole cell lysate

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Predicted band size: 14 kDa

  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Blocking/Diluting buffer 5% NFDM /TBST

    All lanes: Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein/vascular endothelium) whole cell lysate at 20 µg

    Lane 2: HT-1080 (human fibrosarcoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa, 70 kDa

    Observed band size: 110 kDa, 14-17 kDa

  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Blocking/Diluting buffer 5% NFDM /TBST

    All lanes: Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

    Lane 2: L6 (rat skeletal muscle) whole cell lysate at 20 µg

    Lane 3: C2C12 (mouse muscle) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14-17 kDa

  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365), expandable thumbnail

    Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    All lanes: Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

    Lane 1: HT-29 cell lysate at 10 µg

    Lane 2: HUVEC cell lysate at 10 µg

    Lane 3: HT-1080 cell lysate at 10 µg

    Predicted band size: 14 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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