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AB288666

Anti-TWEAKR/FN14 antibody [ITEM-4]

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Mouse Monoclonal TWEAKR/FN14 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD266, FN14, TNFRSF12A, Tumor necrosis factor receptor superfamily member 12A, Fibroblast growth factor-inducible immediate-early response protein 14, Tweak-receptor, FGF-inducible 14, TweakR

2 Images
Flow Cytometry - Anti-TWEAKR/FN14 antibody [ITEM-4] (AB288666)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TWEAKR/FN14 antibody [ITEM-4] (AB288666)

Flow cytometry surface staining pattern of HUVEC cells stained using ab288666 (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Flow Cytometry - Anti-TWEAKR/FN14 antibody [ITEM-4] (AB288666)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TWEAKR/FN14 antibody [ITEM-4] (AB288666)

Separation of HUVEC cells stained using ab288666 (low endotoxin, concentration in sample 1 μg/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ITEM-4

Isotype

IgG2b

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody ITEM-4 recognizes an extracellular epitope of CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 g/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TWEAKR/FN14 known also as FN14 protein is a receptor primarily involved in cellular signaling. It is found on the cell surface and has a molecular weight of approximately 14 kDa. This receptor expresses in various tissues but its expression is especially high in the liver kidney and muscle tissues. Through its engagement with its ligand TWEAK (Tumor Necrosis Factor-Like Weak Inducer of Apoptosis) TWEAKR/FN14 participates in signal transduction processes that regulate cell survival and proliferation.
Biological function summary

TWEAKR/FN14 influences multiple cellular activities including inflammation proliferation and apoptosis. It does not solely function in isolation often interacting with other proteins in complex networks. In fact FN14 often couples its activity with downstream signaling proteins that impact pathways involved in tissue remodeling and repair. The receptor's ability to mediate these processes makes it a significant regulator of tissue response to damage.

Pathways

Signaling through TWEAKR/FN14 primarily involves the NF-κB and MAPK pathways. These signaling pathways are critical for mediating cellular responses to external stimuli such as stress or injury. The NF-κB pathway in particular plays a role in immune and inflammatory responses where FN14 through its interaction modulates these responses. Additionally proteins such as TRAF (TNF Receptor Associated Factor) are related to FN14 through these pathways contributing to its signaling functions.

Dysregulation of TWEAKR/FN14 is linked to pathological conditions such as cancer and fibrosis. Overexpression of FN14 has been observed in several malignancies and its interaction with TWEAK has been implicated in tumorigenic processes. Meanwhile in fibrosis FN14 is associated with excessive tissue repair and scarring connected to the accumulation of extracellular matrix proteins. Altered expression levels and activity of FN14 with its partner proteins can contribute to the progression of these diseases highlighting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
See full target information TNFRSF12A
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Product promise

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