Rabbit Polyclonal TWF1/Twinfilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TWF1 aa 50 to C-terminus.
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- International journal of molecular sciences 21:2020The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUgne Gyvyte et. al.PubMed 32708220
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TWF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12792
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
Alternative names
PTK9, TWF1, Twinfilin-1, Protein A6, Protein tyrosine kinase 9
Recommended products
Rabbit Polyclonal TWF1/Twinfilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TWF1 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TWF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12792
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TWF1 also known as Twinfilin-1 or A6-related protein is an actin-binding protein with a molecular mass of about 38 kDa. This protein is expressed in various tissues including skeletal muscle heart and brain. TWF1 playing a role in actin dynamics binds to actin monomers and may affect actin filament turnover. It acts by promoting actin depolymerization and inhibiting actin nucleation contributing to the dynamic remodeling of the actin cytoskeleton.
- Biological function summary
TWF1 is important in regulating cell motility morphology and division by influencing actin filament dynamics. TWF1 does not necessarily exist as part of a complex but its activity interacts with components of the cytoskeleton. By modulating actin dynamics TWF1 influences processes such as endocytosis cellular movement and response to external stimuli.
- Pathways
Actin dynamics mediated by TWF1 is integral to the cytoskeleton organization pathway and the endocytic pathway. Within these pathways it interacts indirectly with proteins like cofilin and profilin which also regulate actin cytoskeleton. Its activity helps cells to adapt their shape and movement facilitating diverse cellular functions and signaling processes.
- Associated diseases and disorders
TWF1 has relevance to cancer progression and conditions affecting cell mobility. Abnormal regulation of actin dynamics due to altered TWF1 activity might lead to enhanced cancer cell invasion and metastasis. Additionally TWF1 connects with proteins like ADF/Cofilin regarding cancer-related pathways influencing actin structure remodeling and cell movement in malignant cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
10% SDS PAGE.
All lanes: Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 40 kDa
Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725)
10% SDS PAGE.
All lanes: Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution
All lanes: NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (ab154725)
Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1/Twinfilin-1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.
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