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AB154725

Anti-TWF1/Twinfilin-1 antibody

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(3 Publications)

Rabbit Polyclonal TWF1/Twinfilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TWF1 aa 50 to C-terminus.

View Alternative Names

PTK9, TWF1, Twinfilin-1, Protein A6, Protein tyrosine kinase 9

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (AB154725)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TWF1/Twinfilin-1 antibody (AB154725)

Immunofluorescent analysis, by confocal microscopy, of paraformaldehyde-fixed HeLa cells labeling TWF1/Twinfilin-1 with ab154725 at 1/500 dilution (green). Alpha-tubulin filaments were labeled in red.

Western blot - Anti-TWF1/Twinfilin-1 antibody (AB154725)
  • WB

Unknown

Western blot - Anti-TWF1/Twinfilin-1 antibody (AB154725)

10% SDS PAGE.

All lanes:

Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Western blot - Anti-TWF1/Twinfilin-1 antibody (AB154725)
  • WB

Unknown

Western blot - Anti-TWF1/Twinfilin-1 antibody (AB154725)

10% SDS PAGE.

All lanes:

Western blot - Anti-TWF1/Twinfilin-1 antibody (ab154725) at 1/5000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TWF1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12792

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Complementary Products

Proteins & Peptides

AB131676

Recombinant Human TWF1/Twinfilin-1 protein

SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (AB131676)

  • 0
  • 0
Primary Antibodies

AB198503

Anti-GGCT antibody [EPR17107]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-GGCT antibody [EPR17107] (AB198503)

  • 0
  • 0
Primary Antibodies

AB126626

Anti-N WASP antibody [EPR6959]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-N WASP antibody [EPR6959] (AB126626)

  • 5
  • 2
Primary Antibodies

AB305327

Anti-Yes antibody [1/Yes]

Recombinant|Trial Size

Western blot - Anti-Yes antibody [1/Yes] (AB305327)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TWF1 also known as Twinfilin-1 or A6-related protein is an actin-binding protein with a molecular mass of about 38 kDa. This protein is expressed in various tissues including skeletal muscle heart and brain. TWF1 playing a role in actin dynamics binds to actin monomers and may affect actin filament turnover. It acts by promoting actin depolymerization and inhibiting actin nucleation contributing to the dynamic remodeling of the actin cytoskeleton.
Biological function summary

TWF1 is important in regulating cell motility morphology and division by influencing actin filament dynamics. TWF1 does not necessarily exist as part of a complex but its activity interacts with components of the cytoskeleton. By modulating actin dynamics TWF1 influences processes such as endocytosis cellular movement and response to external stimuli.

Pathways

Actin dynamics mediated by TWF1 is integral to the cytoskeleton organization pathway and the endocytic pathway. Within these pathways it interacts indirectly with proteins like cofilin and profilin which also regulate actin cytoskeleton. Its activity helps cells to adapt their shape and movement facilitating diverse cellular functions and signaling processes.

TWF1 has relevance to cancer progression and conditions affecting cell mobility. Abnormal regulation of actin dynamics due to altered TWF1 activity might lead to enhanced cancer cell invasion and metastasis. Additionally TWF1 connects with proteins like ADF/Cofilin regarding cancer-related pathways influencing actin structure remodeling and cell movement in malignant cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
See full target information TWF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 36:e23176 PubMed35968984

2022

Effect of long noncoding RNA CCAT2 on drug sensitivity to 5-fluorouracil of breast cancer cells through microRNA-145 meditated by p53.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyun Zhang,Xuedong Wang,Yueping Wang,Daoping Zhou,Huaiguo Wu,Wei Cheng,Qingping Wang,Guopei Zheng,Ji Wang,Juan Gu

International journal of molecular sciences 21: PubMed32708220

2020

The Role of miR-375-3p and miR-200b-3p in Gastrointestinal Stromal Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Ugne Gyvyte,Rokas Lukosevicius,Ruta Inciuraite,Greta Streleckiene,Greta Gudoityte,Justina Bekampyte,Serena Valentini,Violeta Salteniene,Paulius Ruzgys,Saulius Satkauskas,Kristina Zviniene,Juozas Kupcinskas,Jurgita Skieceviciene

Nature neuroscience 20:661-673 PubMed28346452

2017

Purine synthesis promotes maintenance of brain tumor initiating cells in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuxing Wang,Kailin Yang,Qi Xie,Qiulian Wu,Stephen C Mack,Yu Shi,Leo J Y Kim,Briana C Prager,William A Flavahan,Xiaojing Liu,Meromit Singer,Christopher G Hubert,Tyler E Miller,Wenchao Zhou,Zhi Huang,Xiaoguang Fang,Aviv Regev,Mario L Suvà,Tae Hyun Hwang,Jason W Locasale,Shideng Bao,Jeremy N Rich
View all publications
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Product promise

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