AB50581

Anti-Twist antibody

Name: Anti-Twist antibody (ab50581) | Abcam
Brand: Abcam Limited
SKU: AB50581
Price: 480 USD
Availability: InStock
Rating: 4 (14 reviews)

(14 Reviews)

(176 Publications)

Rabbit Polyclonal Twist antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 176 publications. Immunogen corresponding to Synthetic Peptide within Human TWIST1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (AB50581)

ICC/IF

AbReview23922****

Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (AB50581)

ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.

Image courtesy of an anonymous Abreview.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (AB50581)

Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human TWIST1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15672

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.

Biological function summary

Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.

Pathways

Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.

Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator : NPAS2-BMAL1 heterodimer (By similarity).

See full target information TWIST1

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Publications (176)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:20541 PubMed40593322

2025

MIR155HG promotes metastasis and cisplatin resistance of cervical cancer cells by regulating the miR-409-3p and ZEB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Chen,Jing Wang,Youqiang Heng,Yu Guo,Ka Ding,Cailing Ma

Cells 13: PubMed39329736

2024

Impact of RBMS 3 Progression on Expression of EMT Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz Górnicki,Jakub Lambrinow,Monika Mrozowska,Klaudia Krawczyńska,Natalia Staszko,Alicja Kmiecik,Aleksandra Piotrowska,Agnieszka Gomułkiewicz,Hanna Romanowicz,Beata Smolarz,Marzena Podhorska-Okołów,Jędrzej Grzegrzółka,Agnieszka Rusak,Piotr Dzięgiel

Nature cell biology 26:1470-1481 PubMed39009641

2024

Zeb1 mediates EMT/plasticity-associated ferroptosis sensitivity in cancer cells by regulating lipogenic enzyme expression and phospholipid composition.

Applications

Unspecified application

Species

Unspecified reactive species

Annemarie Schwab,Zhigang Rao,Jie Zhang,André Gollowitzer,Katharina Siebenkäs,Nino Bindel,Elisabetta D'Avanzo,Ruthger van Roey,Yussuf Hajjaj,Ece Özel,Isabell Armstark,Leonhard Bereuter,Fengting Su,Julia Grander,Ehsan Bonyadi Rad,Arwin Groenewoud,Felix B Engel,George W Bell,Whitney S Henry,José Pedro Friedmann Angeli,Marc P Stemmler,Simone Brabletz,Andreas Koeberle,Thomas Brabletz

Journal of cellular physiology 239:e31248 PubMed38501506

2024

Naringin activates semaphorin 3A to ameliorate TGF-β-induced endothelial-to-mesenchymal transition related to atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ju Lai,Shang-Hung Chang,Ying-Chang Tung,Gwo-Jyh Chang,Celina De Almeida,Wei-Jan Chen,Yung-Hsin Yeh,Feng-Chun Tsai

Cell death & disease 15:215 PubMed38485986

2024

Hypoxia-induced miR-5100 promotes exosome-mediated activation of cancer-associated fibroblasts and metastasis of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuansheng Duan,Mengqian Zhou,Beibei Ye,Kai Yue,Feng Qiao,Yuxuan Wang,Qingchuan Lai,Yue Wu,Jiayan Cao,Yansheng Wu,Xudong Wang,Chao Jing

International journal of molecular sciences 25: PubMed38256193

2024

Evaluation of the Therapeutic Effects of Harmine on Anaplastic Thyroid Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Enke Baldini,Silvia Cardarelli,Antonio Francesco Campese,Eleonora Lori,Poupak Fallahi,Camilla Virili,Flavio Forte,Daniele Pironi,Filippo Maria Di Matteo,Piergaspare Palumbo,Maria Ludovica Costanzo,Vito D'Andrea,Marco Centanni,Salvatore Sorrenti,Alessandro Antonelli,Salvatore Ulisse

Cell reports 42:112646 PubMed37314929

2023

Breast cancer cell mesenchymal transition and metastasis directed by DAP5/eIF3d-mediated selective mRNA translation.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Alard,Olga Katsara,Tiffany Rios-Fuller,Columba de la Parra,Ugur Ozerdem,Amanda Ernlund,Robert J Schneider

Surgery today 53:633-639 PubMed36764935

2023

TWIST1 is a prognostic factor for neoadjuvant chemotherapy for patients with resectable pancreatic cancer: a preliminary study.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Fujiwara,Yuriko Saiki,Shinichi Fukushige,Mie Yamanaka,Masaharu Ishida,Fuyuhiko Motoi,Michiaki Unno,Akira Horii

CNS neuroscience & therapeutics 29:878-892 PubMed36382346

2022

Anti-cancer effect of targeting fibroblast activation protein alpha in glioblastoma through remodeling macrophage phenotype and suppressing tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yazhou Miao,Yuxuan Deng,Jinqiu Liu,Jing Wang,Boyi Hu,Shuyu Hao,Herui Wang,Zhe Zhang,Zeping Jin,Yang Zhang,Chunzhao Li,Peng Zhang,Hong Wan,Shaodong Zhang,Jie Feng,Nan Ji

World journal of gastrointestinal oncology 14:1874-1886 PubMed36187399

2022

Prognostic and clinicopathological value of Twist expression in esophageal cancer: A meta-analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Peng Song,Su-Yan Wang,Si-Cheng Zhou,Dong-Sheng Wu,Jia-Yu Xie,Tong-Tong Liu,Xiu-Zhu Wu,Guo-Wei Che

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Twist antibody

Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (AB50581)

Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (AB50581)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (176)

Product promise