Rabbit Polyclonal Twist antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 156 publications. Immunogen corresponding to Synthetic Peptide within Human TWIST1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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- Surgery today 53:633-6392023TWIST1 is a prognostic factor for neoadjuvant chemotherapy for patients with resectable pancreatic cancer: a preliminary study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSho Fujiwara,Yuriko Saiki,Shinichi Fukushige,Mie Yamanaka,Masaharu Ishida,Fuyuhiko Motoi,Michiaki Unno,Akira HoriiPubMed 36764935
- World journal of gastrointestinal oncology 14:1874-18862022Prognostic and clinicopathological value of Twist expression in esophageal cancer: A meta-analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Peng Song,Su-Yan Wang,Si-Cheng Zhou,Dong-Sheng Wu,Jia-Yu Xie,Tong-Tong Liu,Xiu-Zhu Wu,Guo-Wei ChePubMed 36187399
- Frontiers in pharmacology 13:9643702022Shenkang injection improves chronic kidney disease by inhibiting multiple renin-angiotensin system genes by blocking the Wnt/β-catenin signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Ni Wang,Hong-Jiao Liu,Li-Li Ren,Ping Suo,Liang Zou,Ya-Mei Zhang,Xiao-Yong Yu,Ying-Yong ZhaoPubMed 36059935
- British journal of cancer 127:1239-12532022LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Xie,Kunpeng Du,Wei Liu,Chunshan Liu,Baiyao Wang,Yunhong Tian,Rong Li,Xiaoting Huang,Jie Lin,Haifeng Jian,Jian Zhang,Yawei YuanPubMed 35864158
- American journal of cancer research 12:1337-13712022Time-extended exposure of gastric epithelial cells to secretome of -activated fibroblasts induces reprogramming of gastric epithelium towards pre-cancerogenic and pro-invasive phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGracjana Krzysiek-Maczka,Aneta Targosz,Tomasz Wrobel,Milena Paw,Urszula Szczyrk,Janusz Opila,Malgorzata Strzalka,Mateusz Wierdak,Piotr Major,Tomasz Brzozowski,Jarosław Czyz,Agata Ptak-BelowskaPubMed 35411238
- Cancers 13:2021Impact of Epithelial-Mesenchymal Transition on the Immune Landscape in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatima-Zohra Khadri,Marianne Samir Makboul Issac,Louis Arthur GabouryPubMed 34680248
- Chinese medical journal 134:2091-21012021LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Xia,Li-Jun Duan,Bi-Nan Lu,Yu-Zhou Pang,Zong-Ran PangPubMed 34334630
- Journal of experimental & clinical cancer research : CR 40:2142021Integrin alpha-V is an important driver in pancreatic adenocarcinoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarius Kemper,Alina Schiecke,Hanna Maar,Sergey Nikulin,Andrey Poloznikov,Vladimir Galatenko,Michael Tachezy,Florian Gebauer,Tobias Lange,Kristoffer Riecken,Alexander Tonevitsky,Achim Aigner,Jakob Izbicki,Udo Schumacher,Daniel WickleinPubMed 34174926
- Frontiers in oncology 11:6288142021Mutant p53 and Twist1 Co-Expression Predicts Poor Prognosis and Is an Independent Prognostic Factor in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Qu Zhang,Fan Zhang,Yun-Zhu Zeng,Min Chen,Wen-He Huang,Jun-Dong Wu,Wei-Ling Chen,Wen-Liang Gao,Jing-Wen Bai,Rui-Qin Yang,Huan-Cheng Zeng,Xiao-Long Wei,Guo-Jun ZhangPubMed 34249678
- Nature communications 12:38962021Single cell derived mRNA signals across human kidney tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew D Young,Thomas J Mitchell,Lars Custers,Thanasis Margaritis,Francisco Morales-Rodriguez,Kwasi Kwakwa,Eleonora Khabirova,Gerda Kildisiute,Thomas R W Oliver,Ronald R de Krijger,Marry M van den Heuvel-Eibrink,Federico Comitani,Alice Piapi,Eva Bugallo-Blanco,Christine Thevanesan,Christina Burke,Elena Prigmore,Kirsty Ambridge,Kenny Roberts,Felipe A Vieira Braga,Tim H H Coorens,Ignacio Del Valle,Anna Wilbrey-Clark,Lira Mamanova,Grant D Stewart,Vincent J Gnanapragasam,Dyanne Rampling,Neil Sebire,Nicholas Coleman,Liz Hook,Anne Warren,Muzlifah Haniffa,Marcel Kool,Stefan M Pfister,John C Achermann,Xiaoling He,Roger A Barker,Adam Shlien,Omer A Bayraktar,Sarah A Teichmann,Frank C Holstege,Kerstin B Meyer,Jarno Drost,Karin Straathof,Sam BehjatiPubMed 34162837
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TWIST1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q15672
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Chicken | Predicted |
| Chimpanzee | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer (By similarity).
Alternative names
BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38
Recommended products
Rabbit Polyclonal Twist antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 156 publications. Immunogen corresponding to Synthetic Peptide within Human TWIST1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TWIST1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q15672
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.
- Biological function summary
Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.
- Pathways
Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.
- Associated diseases and disorders
Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (ab50581)
Human melanoma Mel15 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 2 µg/ml ab50581.
The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Image courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody (ab50581)
ab50581 staining Twist in human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA for 20 minutes. Samples were then incubated with primary antibody at 1/50 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Cy3® used at a 1/400 dilution.
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