AB314056

Anti-TWIST antibody [BLR156J] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal Twist antibody. Suitable for ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TWIST1 aa 1-50.

View Alternative Names

BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38

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Supplier Data

Immunocytochemistry - Anti-TWIST antibody [BLR156J] - BSA free (AB314056)

Detection of human TWIST1 in FFPE SK-N-BE(2) cells by immunocytochemistry Antibody : Rabbit anti-TWIST1 recombinant monoclonal antibody [BLR156J] (A700-156 lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG . Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-TWIST antibody [BLR156J] - BSA free (AB314056)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-SH cells prepared using NETN lysis buffer. Antibodies : Rabbit antiTWIST1 recombinant monoclonal antibody [BLR156J] used for IP at 20 µl/mg lysate. TWIST1 was also immunoprecipitated by a second antibody against a different epitope of TWIST1 (Antibody 2). For blotting immunoprecipitated TWIST1, A700-156 was used at 1 : 1000. Chemiluminescence with an exposure time of 10 seconds.

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Supplier Data

Western blot - Anti-TWIST antibody [BLR156J] - BSA free (AB314056)

Samples : Whole cell lysate (50 µg) from SK-N-SH, HEK293T, SK-NBE(2), HeLa, and SK-N-MC cells prepared using NETN lysis buffer. Antibody : Rabbit anti-TWIST1 recombinant monoclonal antibody [BLR156J] (A700-156 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-COPB2 antibody (A304-523A).

All lanes:

Western blot - Anti-TWIST antibody [BLR156J] - BSA free (ab314056) at 1/1000 dilution

Lane 1:

SK-N-SH Whole cell lysate at 50 µg

Lane 2:

HEK-293T Whole cell lysate at 50 µg

Lane 3:

SK-NBE(2) Whole cell lysate at 50 µg

Lane 4:

HeLa Whole cell lysate at 50 µg

Lane 5:

SK-N-MC Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR156J

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IP

applications

Immunogen

Synthetic Peptide within Human TWIST1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15672

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.

Biological function summary

Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.

Pathways

Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.

Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator : NPAS2-BMAL1 heterodimer (By similarity).

See full target information TWIST1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TWIST antibody [BLR156J] - BSA free

Immunocytochemistry - Anti-TWIST antibody [BLR156J] - BSA free (AB314056)

Immunoprecipitation - Anti-TWIST antibody [BLR156J] - BSA free (AB314056)

Western blot - Anti-TWIST antibody [BLR156J] - BSA free (AB314056)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise