Anti-Twist antibody [RP23040206]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [RP23040206] (AB313483)

Immunofluorescent analysis of Ntera2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous TWIST1 with ab313483 at 2µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of TWIST1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TWIST1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-Twist Antibody [RP23040206] (AB313483)

All lanes:

Western blot - Anti-Twist Antibody [RP23040206] (ab313483) at 1 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

Jurkat cell lysate at 30 µg

Lane 3:

MOLT4 cell lysate at 30 µg

Lane 4:

Raji cell lysate at 30 µg

Lane 5:

Ramos cell lysate at 30 µg

Lane 6:

K562 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

Observed band size: 25 kDa

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