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Rabbit Recombinant Monoclonal Twist antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TWIST1.

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Images

Western blot - Anti-Twist Antibody [RP23040206] (AB313483), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Twist Antibody [RP23040206] (AB313483), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human TWIST1. Database link Q15672

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
2 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Target data

Function

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer (By similarity).

Alternative names

BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38

Recommended products

Rabbit Recombinant Monoclonal Twist antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TWIST1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human TWIST1. Database link Q15672
Clone number
RP23040206
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.

Biological function summary

Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.

Pathways

Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.

Associated diseases and disorders

Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.

Product promise

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2 product images

  • Western blot - Anti-Twist Antibody [RP23040206] (ab313483), expandable thumbnail

    Western blot - Anti-Twist Antibody [RP23040206] (ab313483)

    All lanes: Western blot - Anti-Twist Antibody [RP23040206] (ab313483) at 1 µg/mL

    Lane 1: HeLa cell lysate at 30 µg

    Lane 2: Jurkat cell lysate at 30 µg

    Lane 3: MOLT4 cell lysate at 30 µg

    Lane 4: Raji cell lysate at 30 µg

    Lane 5: Ramos cell lysate at 30 µg

    Lane 6: K562 cell lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Observed band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Twist Antibody [RP23040206] (ab313483), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Twist Antibody [RP23040206] (ab313483)

    Immunofluorescent analysis of Ntera2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous TWIST1 with ab313483 at 2 ?g/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of TWIST1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TWIST1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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