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Mouse Monoclonal Twist antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Cited in 243 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TWIST1.
Alternative names=Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38, TWIST1, TWIST, BHLHA38
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Fragment Protein within Human TWIST1. The exact immunogen used to generate this antibody is proprietary information. Database link: Q15672
Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
Images
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-100.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.20000-2.00000 µg/mL | Notes Abcam recommends using BSA blocking. |
Species Rat | Dilution info 0.20000-2.00000 µg/mL | Notes Abcam recommends using BSA blocking. |
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes Abcam recommends using BSA blocking. |
Target data
Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).
Recommended products
Mouse Monoclonal Twist antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Cited in 243 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TWIST1.
Alternative names=Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38, TWIST1, TWIST, BHLHA38
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Fragment Protein within Human TWIST1. The exact immunogen used to generate this antibody is proprietary information. Database link: Q15672
- Clone number
Twist2C1a
- Purification technique
Affinity purification Protein G
- Specificity
Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react.
Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC.- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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