Anti-Twist antibody [Twist2C1a]

• WB

Supplier Data

Western blot - Anti-Twist antibody [Twist2C1a] (AB50887)

All lanes:

Western blot - Anti-Twist antibody [Twist2C1a] (ab50887) at 2 µg/mL

Lane 1:

A2058 (Human melanoma cell line) whole cell lysate at 25 µg

Lane 2:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 25 µg

Lane 3:

F2408 (Rat normal fibroblast cell line) whole cell lysate at 25 µg

Predicted band size: 21 kDa

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• ICC

Supplier Data

Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (AB50887)

4% paraformaldehyde-fixed, 0.1% Triton-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Twist using ab50887 at 100 μg/ml in immunocytochemistry.

• ICC

Unknown

Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (AB50887)

ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor^®488 Goat anti-mouse IgG at 1/200 dilution.

• ICC

AbReview20547****

Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (AB50887)

ab50887 staining Twist in a rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• ICC

PubMed

Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (AB50887)

Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody

Loebel DA et al., PLoS One, 9, e98945, 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Unknown

Western blot - Anti-Twist antibody [Twist2C1a] (AB50887)

SDS-PAGE with 10-20% gradient gel.

Lane 1:

Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

Lanes 2 - 5:

Western blot - Anti-Twist antibody [Twist2C1a] (ab50887) at 1/50 dilution

Lane 1:

Marker lane

Lane 2:

Hela whole cell lysate, 25µg

Lane 3:

A2058 whole cell lysate, 25µg

Lane 4:

NIH3T3 whole cell lysate, 25µg

Lane 5:

F2408 whole cell lysate, 25µg

Predicted band size: 21 kDa

Observed band size: 25 kDa

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Exposure time: 2min

• WB

AbReview19099****

Western blot - Anti-Twist antibody [Twist2C1a] (AB50887)

All lanes : Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C
Lanes 2, 3, 5, 6, 8 & 9 : Whole cell lysate of mouse pancreatic tumor
Lanes 1, 4 & 7 : Whole cell lysate of Mouse metastatic pancreatic carcinoma
Lysates at 50 μg per lane
Secondary Antibody : An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique
Blocking Step : 10% Milk for 1 hour at room temperature

All lanes:

Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

Predicted band size: 21 kDa

Observed band size: 100 kDa,25 kDa,65 kDa,75 kDa

false

This image is courtesy of an Abreview submitted by Pawel Mazur