Anti-Twist antibody [Twist2C1a]

7 product images

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  Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

  All lanes: Western blot - Anti-Twist antibody [Twist2C1a] (ab50887) at 2 µg/mL

  Lane 1: A2058 (Human melanoma cell line) whole cell lysate at 25 µg

  Lane 2: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 25 µg

  Lane 3: F2408 (Rat normal fibroblast cell line) whole cell lysate at 25 µg

  Predicted band size: 21 kDa

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  Loebel DA et al., PLoS One, 9, e98945, 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)

  Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody

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  Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)

  4% paraformaldehyde-fixed, 0.1% Triton-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Twist using ab50887 at 100 μg/ml in immunocytochemistry.

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  This image is courtesy of a customer review submitted by Pawel Mazur

  Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

  All lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C
  
  Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
  Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma
  
  Lysates at 50 µg per lane
  
  Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique
  
  
  Blocking Step: 10% Milk for 1 hour at room temperature

  All lanes: Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

  Predicted band size: 21 kDa

  Observed band size: 100 kDa, 25 kDa, 65 kDa, 75 kDa

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  Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)

  ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor^®488 Goat anti-mouse IgG at 1/200 dilution.

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  This image is courtesy of an anonymous customer review

  Immunocytochemistry - Anti-Twist antibody [Twist2C1a] (ab50887)

  ab50887 staining Twist in a rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.

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  Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

  SDS-PAGE with 10-20% gradient gel.

  Lane 1: Western blot - Anti-Twist antibody [Twist2C1a] (ab50887)

  Lanes 2 - 5: Western blot - Anti-Twist antibody [Twist2C1a] (ab50887) at 1/50 dilution

  Lane 1: Marker lane

  Lane 2: Hela whole cell lysate, 25µg

  Lane 3: A2058 whole cell lysate, 25µg

  Lane 4: NIH3T3 whole cell lysate, 25µg

  Lane 5: F2408 whole cell lysate, 25µg

  Predicted band size: 21 kDa

  Observed band size: 25 kDa

  Exposure time: 2min