AB187008

Anti-Twist (phospho S68) antibody [EP18178]

Name: Anti-Twist (phospho S68) antibody [EP18178] (ab187008) | Abcam
Brand: Abcam Limited
SKU: AB187008
Price: 185 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal Twist phospho S68 antibody. Suitable for IP, Dot, WB and reacts with Human samples. Cited in 9 publications.

View Alternative Names

BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38

3 Images

Supplier Data

Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Twist (phospho S68) was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate with ab187008 at 1/60 dilution.

Western blot was performed from the immunoprecipitate using ab187008 at 1/5000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate 10ug (Input).

Lane 2 : ab187008 IP in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab187008 in HEK-293T transfected with GFP-tagged Twist (WT) whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (ab187008)

Predicted band size: 21 kDa

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Supplier Data

Western blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Blocking/Dilution buffer : 5% NFDM/TBST.

PMID : 21502402.

All lanes:

Western blot - Anti-Twist (phospho S68) antibody [EP18178] (ab187008) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT) whole cell lysate at 10 µg

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney) transfected with GFP-tagged Twist (WT), then treated with alkaline phosphatase for 1 hour, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 47 kDa

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Exposure time: 10s

Supplier Data

Dot Blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Dot blot analysis of Twist (phospho S68) labeled with ab187008 at 1/1000 dilution.

Lane 1 : Twist (phospho S68) peptide;

Lane 2 : Twist non-phospho peptide.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-Twist (phospho S68) antibody [EP18178] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP18178

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.

Biological function summary

Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.

Pathways

Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.

Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator : NPAS2-BMAL1 heterodimer (By similarity).

See full target information TWIST1 pS68

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 14:584-594 PubMed39974382

2025

LncRNA ZEB1-AS1 promotes the proliferation and migration of non-small cell lung cancer by activating epithelial-mesenchymal transition with STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengjin Chen,Yangwei Yao,Jingpeng Gao

Development (Cambridge, England) 149: PubMed35781329

2022

TWIST1 interacts with β/δ-catenins during neural tube development and regulates fate transition in cranial neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica W Bertol,Shelby Johnston,Rabia Ahmed,Victoria K Xie,Kelsea M Hubka,Lissette Cruz,Larissa Nitschke,Marta Stetsiv,Jeremy P Goering,Paul Nistor,Sally Lowell,Hanne Hoskens,Peter Claes,Seth M Weinberg,Irfan Saadi,Mary C Farach-Carson,Walid D Fakhouri

Cell death discovery 8:2 PubMed35013126

2022

MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation.

Applications

Species

Rat

Jingzhang Fan,Shiming Li,Dawei Wang

Cell death & disease 12:349 PubMed33824282

2021

ZEB1-induced LINC01559 expedites cell proliferation, migration and EMT process in gastric cancer through recruiting IGF2BP2 to stabilize ZEB1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Huojian Shen,Hongyi Zhu,Yuanwen Chen,Zhiyong Shen,Weiqing Qiu,Changlin Qian,Jie Zhang

Technology in cancer research & treatment 20:1533033821990007 PubMed33550957

2021

Silencing of Long Non-Coding RNA FGD5-AS1 Inhibits the Progression of Non-Small Cell Lung Cancer by Regulating the miR-493-5p/DDX5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Cui,Peng Luo,Yao Bai,Jiangping Meng

Cancer management and research 12:6707-6717 PubMed32801903

2020

OIP5-AS1/miR-137/ZNF217 Axis Promotes Malignant Behaviors in Epithelial Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Guo,Jiabao Chen,Dong Liu,Lili Liu

Biochemical pharmacology 180:114152 PubMed32679125

2020

α-Linolenic acid inhibits the migration of human triple-negative breast cancer cells by attenuating Twist1 expression and suppressing Twist1-mediated epithelial-mesenchymal transition.

Applications

Species

Human

Shih-Chung Wang,Hai-Lun Sun,Yi-Hsuan Hsu,Shu-Hui Liu,Chong-Kuei Lii,Chia-Han Tsai,Kai-Li Liu,Chin-Shiu Huang,Chien-Chun Li

International journal of oncology 57:858-870 PubMed32582960

2020

GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Cheng,Lanying Gou,Ting Wei,Jian Zhang

Cell proliferation 53:e12823 PubMed32515533

2020

LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xu,Baohua Yang,Lifeng Wang,Yunheng Zhu,Xiuxiang Zhu,Ziyin Xia,Zhen Zhao,Ling Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Twist (phospho S68) antibody [EP18178]

Immunoprecipitation - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Western blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Dot Blot - Anti-Twist (phospho S68) antibody [EP18178] (AB187008)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise