Rabbit Polyclonal Twist2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Recombinant full length protein - Human samples. Cited in 25 publications.
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- Cell proliferation 56:e134592023Adult progenitor rejuvenation with embryonic factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Wang,Xupeng Liu,Yu Chen,Elwin Tan Jun-Hao,Ziyue Yao,Jason Chua Min-Wen,Benjamin Chua Yan-Jiang,Shilin Ma,Wenwu Ma,Lanfang Luo,Luyao Guo,Dan Song,Ng Shyh-ChangPubMed 37177849
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e226422022Twist2-expressing cells reside in human skeletal muscle and are responsive to aging and resistance exercise training.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNick Gaulton,Griffen Wakelin,Laura V Young,Scott Wotherspoon,Michael Kamal,Gianni Parise,Joshua P Nederveen,Andy Holwerda,Lex B Verdijk,Luc J C van Loon,Tim Snijders,Adam P JohnstonPubMed 36374263
- BioMed research international 2022:28251722022The Elevated Circ_0067835 Could Accelerate Cell Proliferation and Metastasis via miR-1236-3p/Twist2 Axis in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianghong Chen,Zihan QiPubMed 36262967
- International archives of allergy and immunology 183:1098-11132022Twist2 Reduced NLRP3-Induced Inflammation of Infantile Pneumonia via Regulation of Mitochondrial Permeability Transition by FOXO1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNiu Ding,Dian Liu,Xiaojun Duan,Jin Zhang,Song Ma,Yanping ChenPubMed 35700708
- Cell reports 39:1108212022Twist2-driven chromatin remodeling governs the postnatal maturation of dermal fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Yong Kim,Minji Park,Jungyoon Ohn,Rho Hyun Seong,Jin Ho Chung,Kyu Han Kim,Seong Jin Jo,Ohsang KwonPubMed 35584664
- Frontiers in oncology 11:7956332022PRL1 Promotes Glioblastoma Invasion and Tumorigenesis Activating USP36-Mediated Snail2 Deubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjin Qiu,Xiaomin Cai,Kaya Xu,Shibin Song,Zumu Xiao,Yunan Hou,Xiaolan Qi,Feng Liu,Yimin Chen,Hua Yang,Liangzhao Chu,Jian LiuPubMed 35111679
- Frontiers in cell and developmental biology 9:7423192021An E2F5-TFDP1-BRG1 Complex Mediates Transcriptional Activation of MYCN in Hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiwen Fan,Ming Kong,Xiulian Miao,Yan Guo,Haozhen Ren,Jinglin Wang,Shuai Wang,Ning Tang,Longcheng Shang,Zhengyi Zhu,Hanyi Liu,Wei Zhu,Xiaolei ShiPubMed 34746136
- Aging 12:25060-250752020LINC01235-TWIST2 feedback loop facilitates epithelial-mesenchymal transition in gastric cancer by inhibiting THBS2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-En Tan,Yao Xing,Ban-Lai Ran,Chao Zhang,Si-Wei Pan,Wen An,Qing-Chuan Chen,Hui-Mian XuPubMed 33206629
- Scientific reports 10:188292020Twist2 is NFkB-responsive when p120-catenin is inactivated and EGFR is overexpressed in esophageal keratinocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeather L Lehman et. al.PubMed 33139779
- Cell death and differentiation 27:3053-30642020Twist2 promotes CD8 T-cell differentiation by repressing ThPOK expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunsook Hwang,Changjin Lee,Kyungsoo Park,Sangwook Oh,Shin Jeon,Byeonggeun Kang,Yehyun Kim,Jaehak Oh,Sung Ho Jeon,Masanobu Satake,Ichiro Taniuchi,Ho Lee,Rho Hyun SeongPubMed 32424141
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Recombinant full length protein - Human | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Cow | Dilution info - | Notes - |
Target data
Function
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity). Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.
Alternative names
Twist-related protein 2, Class A basic helix-loop-helix protein 39, Dermis-expressed protein 1, bHLHa39, Dermo-1, DERMO1, BHLHA39, TWIST2
Recommended products
Rabbit Polyclonal Twist2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Recombinant full length protein - Human samples. Cited in 25 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody is able to detect both Twist1 and Twist2 proteins. We have observed weak detection of the Twist1 protein using Twist1 recombinant proteins along with select lysates generating a BOI of weak signal associated to Twist1. The signal detected in the endogenous lysates and recombinant proteins is much greater for Twist2.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-Twist2 antibody (ab66031)
This antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater. The BOI at 18kDa is related to the higher affinity binding of Twist2. In select lysates, we have observed a weaker band between 21-25kDa likely to be the cross reactivity of Twist1.
All lanes: Western blot - Anti-Twist2 antibody (ab66031) at 1 µg/mL
Lane 1: Human liver tissue lysate - total protein (ab29889) at 10 µg
Lane 2: Human small intestine tissue lysate - total protein (ab29276) at 10 µg
Lane 3: Human testis tissue lysate - total protein (ab30257) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa, 24 kDa, 55 kDa, 90 kDa
Immunoprecipitation - Anti-Twist2 antibody (ab66031)
Twist2 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Twist2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab66031.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 18kDa; Twist2All lanes: Immunoprecipitation - Anti-Twist2 antibody (ab66031)
Predicted band size: 18 kDa
Western blot - Anti-Twist2 antibody (ab66031)
This antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater. The BOI at 18kDa is related to the higher affinity binding of Twist2. In select lysates, we have observed a weaker band between 21-25kDa likely to be the cross reactivity of Twist1.
All lanes: ab66031 at 1 µg/mL
Lane 1: Human liver tissue lysate at 10 µg
Lane 2: Human HEK293 whole cell lysate at 10 µg
Lane 3: Human T47D whole cell lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 3000 Cells
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 21 kDa, 45 kDa, 46 kDa
Exposure time: 1min
Western blot - Anti-Twist2 antibody (ab66031)
This antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater, as demonstrated by the use of the recombinant proteins.
All lanes: ab66031 at 1 µg/mL
Lane 1: Recombinant Human Twist2 protein ab132641 at 0.1 µg
Lane 2: Recombinant Human Twist2 protein ab153585 at 0.1 µg
Lane 3: Recombinant Human Twist protein ab112368 at 0.1 µg
Lane 4: Recombinant Human Twist protein Recombinant Human Twist protein ab132349 at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 37 kDa, 45 kDa, 48 kDa
Exposure time: 4min
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