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AB244263

Anti-TXNDC/TMX antibody

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(2 Publications)

Rabbit Polyclonal TXNDC/TMX antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TMX1 aa 200 to C-terminus.

View Alternative Names

TMX, TXNDC, TXNDC1, PSEC0085, UNQ235/PRO268, TMX1, Thioredoxin-related transmembrane protein 1, Protein disulfide-isomerase TMX1, Thioredoxin domain-containing protein 1, Transmembrane Trx-related protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC/TMX antibody (AB244263)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC/TMX antibody (AB244263)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TXNDC/TMX (green) using ab244263 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC/TMX antibody (AB244263)

Formalin-fixed, paraffin-embedded human testis tissue stained for TXNDC/TMX using ab244263 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-TXNDC/TMX antibody (AB244263)
  • WB

Unknown

Western blot - Anti-TXNDC/TMX antibody (AB244263)

All lanes:

Western blot - Anti-TXNDC/TMX antibody (ab244263) at 0.4 µg/mL

All lanes:

CAPAN-2 cell lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TMX1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H3N1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC also known as TMX or thioredoxin domain-containing protein is a protein with enzymatic properties. This protein functions as a disulfide isomerase and is involved in the formation and rearrangement of disulfide bonds within proteins. TXNDC weighs around 38 kDa and localizes primarily in the endoplasmic reticulum (ER). TXNDC facilitates oxidative protein folding by catalyzing thiol-disulfide exchange reactions. Its expression is common in tissues with high protein synthesis demand such as liver and pancreas.
Biological function summary

Thioredoxin domain-containing protein plays a significant role in maintaining cellular redox homeostasis. It forms part of a complex network with other oxidoreductases contributing to oxidative protein folding processes essential for proper protein maturation and function. Beyond protein folding TXNDC participates in the detoxification of reactive oxygen species helping protect cells from oxidative stress. This function supports normal cellular metabolism and survival under potentially damaging conditions.

Pathways

TXNDC is active within the oxidative protein folding pathway which involves the transfer of electrons from substrate proteins to the molecular oxidant. It collaborates closely with protein disulfide isomerase (PDI) in this pathway to ensure proper protein structure and function. Another significant pathway includes its involvement in the unfolded protein response (UPR) a mechanism that manages protein misfolding stress in the ER. TXNDC modulates the UPR by influencing the levels of reactive oxygen species indirectly impacting the function of pathways regulated by proteins such as IRE1 and PERK.

Alterations in TXNDC function have connections to several pathological conditions. One such condition is cancer where imbalances in TXNDC expression levels can contribute to tumor progression and chemoresistance often in coordination with other redox proteins like thioredoxin itself. Additionally improper TXNDC function can relate to neurodegenerative diseases such as Alzheimer's as oxidative stress and protein misfolding are common features. In Alzheimer's TXNDC's involvement in redox regulation and protein homeostasis links it to the broader pathology involving proteins like amyloid-beta and tau.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thiredoxin domain-containing protein that participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions (PubMed : 11152479, PubMed : 37648867). Acts as a key inhibitor of the alternative triglyceride biosynthesis pathway by inhibiting the activity of TMEM68/DIESL at the endoplasmic reticulum, thereby restricting accumulation of triacylglycerol (PubMed : 37648867). The alternative triglyceride biosynthesis pathway mediates formation of triacylglycerol from diacylglycerol and membrane phospholipids (PubMed : 37648867). Acts as a protein disulfide isomerase by catalyzing formation or reduction of disulfide bonds (PubMed : 22228764, PubMed : 29932915). Specifically mediates formation of disulfide bonds of transmembrane proteins at the endoplasmic reticulum membrane (PubMed : 22228764). Involved in endoplasmic reticulum-associated degradation (ERAD) via its protein disulfide isomerase activity by acting on folding-defective polypeptides at the endoplasmic reticulum membrane (PubMed : 29932915). Acts as a negative regulator of platelet aggregation following secretion in the extracellular space (PubMed : 30425049). Acts as a regulator of endoplasmic reticulum-mitochondria contact sites via its ability to regulate redox signals (PubMed : 27502484, PubMed : 31304984). Regulates endoplasmic reticulum-mitochondria Ca(2+) flux (PubMed : 27502484).
See full target information TMX1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:560 PubMed39574183

2024

Exploiting potential molecular compounds for treating testicular seminoma by targeting immune related genes.

Applications

Unspecified application

Species

Unspecified reactive species

Yankang Cui,Xiaodie Zhou,Jing Zhang,Bo Fang,Jingping Ge,Hao Tang,Bianjiang Liu,Haowei He,Feng Xu,Xuejun Shang

BMC genomics 23:363 PubMed35546662

2022

Ewe breed differences in the cervical transcriptome at the follicular phase of a synchronised oestrous cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Abril-Parreño,Kieran G Meade,Anette Kristine Krogenæs,Xavier Druart,Paul Cormican,Sean Fair
View all publications
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Product promise

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