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AB250956

Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free

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Rabbit Recombinant Monoclonal TXNDC11/EFP1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

EFP1, TXNDC11, Thioredoxin domain-containing protein 11, EF-hand-binding protein 1

3 Images
Immunoprecipitation - Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free (AB250956)
  • IP

Supplier Data

Immunoprecipitation - Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free (AB250956)

This data was developed using ab188329, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from LnCaP lysate immunoprecipitated using ab188329 at 1/50 dilution. Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (<a href='/en-us/products/primary-antibodies/txndc11-efp1-antibody-epr14314-c-terminal-ab188329'>ab188329</a>)

Predicted band size: 111 kDa

false

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free (AB250956)
  • WB

Supplier Data

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free (AB250956)

This data was developed using ab188329, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (<a href='/en-us/products/primary-antibodies/txndc11-efp1-antibody-epr14314-c-terminal-ab188329'>ab188329</a>) at 1/1000 dilution

Lane 1:

LnCaP cell lysate at 20 µg

Lane 2:

T.T cell lysate at 20 µg

Lane 3:

PC3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 111 kDa

false

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free (AB250956)
  • WB

Supplier Data

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free (AB250956)

This data was developed using ab188329, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (<a href='/en-us/products/primary-antibodies/txndc11-efp1-antibody-epr14314-c-terminal-ab188329'>ab188329</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 111 kDa

false

  • Unconjugated

    Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14314

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on the sequence analysis, this antibody recognizes 2 isoforms with the predicted MWs of 111KDa and 108KDa, respectively.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250956 is the carrier-free version of ab188329.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC11 also known as EFP1 is a thioredoxin domain-containing protein with a molecular mass of approximately 119 kDa. This protein acts as a disulfide isomerase involved in protein folding and redox regulation. TXNDC11 localizes mostly in the endoplasmic reticulum where its expression helps maintain cellular redox homeostasis. Recent studies also indicate expression in various tissues which suggests a role in broader cellular mechanisms.
Biological function summary

The redox regulation function of TXNDC11 contributes to the maturation and stabilization of nascent proteins. It appears to interact with other proteins in a complex manner to facilitate these processes. TXNDC11 mediates the formation and rearrangement of disulfide bonds serving as a chaperone that assists in proper protein folding which is essential for maintaining cell function and viability.

Pathways

TXNDC11 integrates into the protein processing pathways within the endoplasmic reticulum. It plays a role in the unfolded protein response (UPR) pathway important for managing ER stress. Proteins such as BiP/GRP78 and protein disulfide isomerase (PDI) work in conjunction with TXNDC11 to ensure proper protein folding under stress conditions highlighting its pathway integration.

TXNDC11's malfunction or misexpression links to diseases involving protein misfolding such as neurodegenerative disorders and certain cancers. Its roles in redox mechanisms and ER stress responses also connect it to insulin resistance syndromes. Interactions with proteins like IRE1 and CHOP suggest a significant role in these pathological conditions making it a potential target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a redox regulator involved in DUOX proteins folding. The interaction with DUOX1 and DUOX2 suggest that it belongs to a multiprotein complex constituting the thyroid H(2)O(2) generating system. It is however not sufficient to assist DUOX1 and DUOX2 in H(2)O(2) generation.
See full target information TXNDC11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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