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AB188329

Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal

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(5 Publications)

Rabbit Recombinant Monoclonal TXNDC11/EFP1 antibody. C-terminal. Suitable for IP, WB and reacts with Human, Rat, Mouse samples. Cited in 5 publications.

View Alternative Names

EFP1, TXNDC11, Thioredoxin domain-containing protein 11, EF-hand-binding protein 1

3 Images
Immunoprecipitation - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)
  • IP

Supplier Data

Immunoprecipitation - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)

Western blot analysis of immunoprecipitation pellet from LnCaP lysate immunoprecipitated using ab188329 at 1/50 dilution. Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (ab188329)

Predicted band size: 111 kDa

false

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)
  • WB

Supplier Data

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)

All lanes:

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (ab188329) at 1/1000 dilution

Lane 1:

LnCaP cell lysate at 20 µg

Lane 2:

T.T cell lysate at 20 µg

Lane 3:

PC3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 111 kDa

false

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)
  • WB

Supplier Data

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (AB188329)

All lanes:

Western blot - Anti-TXNDC11/EFP1 antibody [EPR14314] - C-terminal (ab188329) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 111 kDa

false

  • Carrier free

    Anti-TXNDC11/EFP1 antibody [EPR14314] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14314

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on the sequence analysis, ab188329 recognizes 2 isoforms with the predicted MWs of 111KDa and 108KDa, respectively.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC11 also known as EFP1 is a thioredoxin domain-containing protein with a molecular mass of approximately 119 kDa. This protein acts as a disulfide isomerase involved in protein folding and redox regulation. TXNDC11 localizes mostly in the endoplasmic reticulum where its expression helps maintain cellular redox homeostasis. Recent studies also indicate expression in various tissues which suggests a role in broader cellular mechanisms.
Biological function summary

The redox regulation function of TXNDC11 contributes to the maturation and stabilization of nascent proteins. It appears to interact with other proteins in a complex manner to facilitate these processes. TXNDC11 mediates the formation and rearrangement of disulfide bonds serving as a chaperone that assists in proper protein folding which is essential for maintaining cell function and viability.

Pathways

TXNDC11 integrates into the protein processing pathways within the endoplasmic reticulum. It plays a role in the unfolded protein response (UPR) pathway important for managing ER stress. Proteins such as BiP/GRP78 and protein disulfide isomerase (PDI) work in conjunction with TXNDC11 to ensure proper protein folding under stress conditions highlighting its pathway integration.

TXNDC11's malfunction or misexpression links to diseases involving protein misfolding such as neurodegenerative disorders and certain cancers. Its roles in redox mechanisms and ER stress responses also connect it to insulin resistance syndromes. Interactions with proteins like IRE1 and CHOP suggest a significant role in these pathological conditions making it a potential target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a redox regulator involved in DUOX proteins folding. The interaction with DUOX1 and DUOX2 suggest that it belongs to a multiprotein complex constituting the thyroid H(2)O(2) generating system. It is however not sufficient to assist DUOX1 and DUOX2 in H(2)O(2) generation.
See full target information TXNDC11
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4121 PubMed35840578

2022

The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Ian D Ferguson,Bonell Patiño-Escobar,Sami T Tuomivaara,Yu-Hsiu T Lin,Matthew A Nix,Kevin K Leung,Corynn Kasap,Emilio Ramos,Wilson Nieves Vasquez,Alexis Talbot,Martina Hale,Akul Naik,Audrey Kishishita,Priya Choudhry,Antonia Lopez-Girona,Weili Miao,Sandy W Wong,Jeffrey L Wolf,Thomas G Martin,Nina Shah,Scott Vandenberg,Sonam Prakash,Lenka Besse,Christoph Driessen,Avery D Posey,R Dyche Mullins,Justin Eyquem,James A Wells,Arun P Wiita

eLife 10: PubMed34698634

2021

Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD.

Applications

Unspecified application

Species

Unspecified reactive species

Ginto George,Satoshi Ninagawa,Hirokazu Yagi,Jun-Ichi Furukawa,Noritaka Hashii,Akiko Ishii-Watabe,Ying Deng,Kazutoshi Matsushita,Tokiro Ishikawa,Yugoviandi P Mamahit,Yuta Maki,Yasuhiro Kajihara,Koichi Kato,Tetsuya Okada,Kazutoshi Mori

eLife 9: PubMed32065582

2020

EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD.

Applications

Unspecified application

Species

Unspecified reactive species

Ginto George,Satoshi Ninagawa,Hirokazu Yagi,Taiki Saito,Tokiro Ishikawa,Tetsushi Sakuma,Takashi Yamamoto,Koshi Imami,Yasushi Ishihama,Koichi Kato,Tetsuya Okada,Kazutoshi Mori

Journal of clinical medicine 8: PubMed31336913

2019

Leptin Modulates Exosome Biogenesis in Breast Cancer Cells: An Additional Mechanism in Cell-to-Cell Communication.

Applications

Unspecified application

Species

Unspecified reactive species

Cinzia Giordano,Luca Gelsomino,Ines Barone,Salvatore Panza,Giuseppina Augimeri,Daniela Bonofiglio,Daniela Rovito,Giuseppina Daniela Naimo,Antonella Leggio,Stefania Catalano,Sebastiano Andò

Nature communications 7:11786 PubMed27283361

2016

Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens.

Applications

Unspecified application

Species

Unspecified reactive species

Richard T Timms,Sam A Menzies,Iva A Tchasovnikarova,Lea C Christensen,James C Williamson,Robin Antrobus,Gordon Dougan,Lars Ellgaard,Paul J Lehner
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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