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AB174276

Anti-TXNDC13/TMX4 antibody [EPR12321]

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Rabbit Recombinant Monoclonal TXNDC13/TMX4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples.

View Alternative Names

KIAA1162, TXNDC13, PSEC0095, UNQ475/PRO938, TMX4, Thioredoxin-related transmembrane protein 4, Thioredoxin domain-containing protein 13

3 Images
Western blot - Anti-TXNDC13/TMX4 antibody [EPR12321] (AB174276)
  • WB

Supplier Data

Western blot - Anti-TXNDC13/TMX4 antibody [EPR12321] (AB174276)

All lanes:

Western blot - Anti-TXNDC13/TMX4 antibody [EPR12321] (ab174276) at 1/1000 dilution

Lane 1:

A375 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Predicted band size: 39 kDa

false

Immunoprecipitation - Anti-TXNDC13/TMX4 antibody [EPR12321] (AB174276)
  • IP

Supplier Data

Immunoprecipitation - Anti-TXNDC13/TMX4 antibody [EPR12321] (AB174276)

Western blot analysis on immunoprecipitation pellet from (1) A375 cell lysate or (2) 1X PBS (negative control) using ab174276 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TXNDC13/TMX4 antibody [EPR12321] (ab174276)

Predicted band size: 39 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC13/TMX4 antibody [EPR12321] (AB174276)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC13/TMX4 antibody [EPR12321] (AB174276)

Immunofluorescent analysis of A375 cells labeling TXNDC13/TMX4 with ab174276 at 1/50 dilution (red). DAPI nuclear staining (blue).

  • Carrier free

    Anti-TXNDC13/TMX4 antibody [EPR12321] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12321

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC13 also known as TMX4 is a protein involved in redox reactions functioning as a thioredoxin-like enzyme. This protein has a molecular mass of approximately 45 kDa and is located primarily in the endoplasmic reticulum. Expression of TXNDC13/TMX4 is observed in various tissues but it is notably high in ones where protein folding and secretion activities are extensive. The involvement of TXNDC13/TMX4 in protein disulfide isomerization centers around its ability to catalyze the formation and breaking of disulfide bonds in proteins which is essential for proper protein folding and maturation.
Biological function summary

The role of thioredoxin-like proteins such as TXNDC13/TMX4 is critical in maintaining cellular redox balance and participating in oxidative stress response. TXNDC13/TMX4 does not appear to be a part of a larger protein complex but operates by interacting directly with other proteins as a chaperone. It helps in the formation of disulfide bonds and alleviates misfolded proteins ensuring correct protein structure and function. This activity is particularly significant in cells with high secretory demands.

Pathways

TXNDC13/TMX4 is involved in pathways governing cellular redox homeostasis and the unfolded protein response. It acts in the thioredoxin pathway an important regulator of the cellular redox environment. The protein's functioning relates to other proteins such as PDI which are key elements in the protein-folding machinery within the endoplasmic reticulum. TXNDC13/TMX4 works closely with such proteins to manage oxidation and reduction reactions essential for controlling cellular oxidative stress levels.

TXNDC13/TMX4 links with conditions involving oxidative stress and protein misfolding such as neurodegenerative diseases and certain cancers. Dysregulation in this protein's activity can contribute to the aggregation of misfolded proteins exacerbating disorders like neurodegeneration. It may also have a role in tumor biology where altered redox states are often observed. The protein is connected to others like TXN which also influence cellular responses to oxidative stress further implicating it in the pathological mechanisms of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TMX4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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