AB137611

Anti-TXNDC4 antibody

Name: Anti-TXNDC4 antibody (ab137611) | Abcam
Brand: Abcam Limited
SKU: AB137611
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal TXNDC4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ERP44 aa 200 to C-terminus.

View Alternative Names

KIAA0573, TXNDC4, UNQ532/PRO1075, ERP44, Endoplasmic reticulum resident protein 44, ER protein 44, ERp44, Thioredoxin domain-containing protein 4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC4 antibody (AB137611)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC4 antibody (AB137611)

ab137611, at a 1/100 dilution, staining TXNDC4 in paraffin embedded SCC4 xenograft tissue by Immunohistochemistry.

Unknown

Western blot - Anti-TXNDC4 antibody (AB137611)

10% SDS PAGE

All lanes:

Western blot - Anti-TXNDC4 antibody (ab137611) at 1/1000 dilution

Lane 1:

Raji whole cell lysates at 30 µg

Lane 2:

H1299 whole cell lysates at 30 µg

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ERP44 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BS26

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC4 also known as ERp46 or thioredoxin domain-containing protein 4 is an enzyme involved in protein folding within the endoplasmic reticulum. With a molecular mass of approximately 47 kDa this protein exhibits oxidoreductase activity and facilitates the formation and isomerization of disulfide bonds in nascent proteins. TXNDC4 is widely expressed across various tissues with a higher presence in the liver and pancreas reflecting its significant role in protein maturation and quality control processes.

Biological function summary

TXNDC4 influences various cellular mechanisms by maintaining redox homeostasis and promoting the correct folding of proteins. It functions as part of a multi-protein complex interacting with other chaperones and co-chaperones within the endoplasmic reticulum. These interactions are critical for efficient protein maturation preventing the accumulation of misfolded proteins and thereby avoiding cellular stress. TXNDC4 is essential for maintaining the functional integrity of secretory pathways which support numerous cellular functions.

Pathways

TXNDC4 plays a role in the unfolded protein response (UPR) and the oxidative folding pathway. The protein interacts closely with components such as protein disulfide isomerase (PDI) and ER oxidoreductin 1 (Ero1) within these pathways. Together these proteins facilitate efficient protein folding and prevent overaccumulation of unfolded proteins. The activity of TXNDC4 also relates to the regulation of cellular redox conditions which are pivotal for maintaining cellular homeostasis and proper response to stress.

Research indicates a connection between TXNDC4 and several conditions such as cancer and diabetes. Abnormal expression or malfunction of TXNDC4 can contribute to improper protein folding which may lead to unfolded protein response activation enhancing cellular stress conditions associated with these disorders. In cancer interactions between TXNDC4 and other proteins like Ero1α can alter redox balance affecting cell proliferation and survival. In diabetes improper protein processing in insulin-secreting cells may involve TXNDC4 dysfunction impacting insulin secretion and cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif (PubMed : 11847130, PubMed : 14517240). Inhibits the calcium channel activity of ITPR1 (PubMed : 15652484). May have a role in the control of oxidative protein folding in the endoplasmic reticulum (PubMed : 11847130, PubMed : 14517240, PubMed : 29858230). Required to retain ERO1A and ERO1B in the endoplasmic reticulum (PubMed : 11847130, PubMed : 29858230).

See full target information ERP44

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:9561-9574 PubMed34719307

2021

Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Xiaoxia Qiu,Fang Wang

Oncology letters 14:1460-1463 PubMed28789365

2017

Effects and mechanism of GA-13315 on the proliferation and apoptosis of KB cells in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Shen,Jingxia Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TXNDC4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC4 antibody (AB137611)

Western blot - Anti-TXNDC4 antibody (AB137611)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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