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Rabbit Recombinant Monoclonal TXNDC9 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15239] (AB191405), expandable thumbnail
  • Western blot - Anti-TXNDC9 antibody [EPR15239] (AB191405), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TXNDC9 antibody [EPR15239] (AB191405), expandable thumbnail
  • Western blot - Anti-TXNDC9 antibody [EPR15239] (AB191405), expandable thumbnail
  • Western blot - Anti-TXNDC9 antibody [EPR15239] (AB191405), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Significantly diminishes the chaperonin TCP1 complex ATPase activity, thus negatively impacts protein folding, including that of actin or tubulin.

Alternative names

APACD, TXNDC9, Thioredoxin domain-containing protein 9, ATP-binding protein associated with cell differentiation, Protein 1-4

Recommended products

Rabbit Recombinant Monoclonal TXNDC9 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15239
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TXNDC9 also known as Thioredoxin Domain Containing 9 or ERdj5 functions as a protein disulfide isomerase involved in the reduction and isomerization of disulfide bonds. This protein weighs approximately 56 kDa and exhibits expression mostly in the endoplasmic reticulum of various cells. TXNDC9 with its oxidoreductase activity plays an important role in maintaining protein folding homeostasis and cellular redox balance. Researchers have identified it as a central player in protein quality control mechanisms.

Biological function summary

TXNDC9 assists in the protein folding process within the endoplasmic reticulum acting as part of larger protein complexes that include other chaperones. It particularly engages in helping misfolded proteins to attain their correct conformation contributing significantly to the protein folding machinery. Additionally TXNDC9 interacts with client proteins guiding their correct structural organization and preventing the accumulation of misfolded proteins that could be harmful to the cell.

Pathways

TXNDC9 occupies a significant position in the endoplasmic reticulum-associated degradation (ERAD) pathway and the unfolded protein response (UPR) pathway. It interacts with proteins such as Ero1 and BiP to ensure proper degradation of misfolded proteins and maintain cellular stress responses. In the ERAD pathway TXNDC9 acts to identify and prepare proteins for degradation while in the UPR pathway it supports roles that mitigate stress signals caused by accumulated unfolded proteins.

Associated diseases and disorders

TXNDC9 involvement links to neurodegenerative diseases and certain cancers. Its role in maintaining protein homeostasis reflects connections to Alzheimer's disease where protein misfolding aggregates contribute to pathology. Similarly aberrant TXNDC9 function associates with tumor development in some cancers due to its involvement with cellular redox status and growth control mechanisms. In these contexts TXNDC9 may interact with proteins like Amyloid-beta in Alzheimer's and could influence oncogenic pathways through interactions with tumor-suppressor proteins.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15239] (ab191405), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNDC9 antibody [EPR15239] (ab191405)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling TXNDC9 with ab191405 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405), expandable thumbnail

    Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

    Lanes 1-3: Merged signal (red and green). Green - ab191405 observed at 27 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab191405 Anti-TXNDC9 antibody [EPR15239] was shown to specifically react with TXNDC9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TXNDC9 knockout HeLa cell line ab265420 (knockout cell lysate Human TXNDC9 knockout HeLa cell lysate ab258257) was used. Wild-type and TXNDC9 knockout samples were subjected to SDS-PAGE. ab191405 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: TXNDC9 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human TXNDC9 knockout HeLa cell line (Human TXNDC9 knockout HeLa cell line ab265420)

    Lane 3: K-562 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TXNDC9 antibody [EPR15239] (ab191405), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TXNDC9 antibody [EPR15239] (ab191405)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized SW480 cells labeling TXNDC9 with ab191405 at 1/400 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

    The two negative controls used anti-TXNDC9 primary antibody at 1/200 dilution followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405), expandable thumbnail

    Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/10000 dilution

    All lanes: K562 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405), expandable thumbnail

    Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: Raji cell lysate at 20 µg

    Lane 3: SW80 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405), expandable thumbnail

    Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TXNDC9 antibody [EPR15239] (ab191405) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: Raw264.7 cell lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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