Anti-TXNL1 antibody [EPR16061(B)] - N-terminal

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%)labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

Lanes 1-4 : Merged signal (red and green). Green - ab188328 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.

ab188328 Anti-TXNL1 antibody [EPR16061(B)] - N-terminal was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TXNL1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TXNL1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-txnl1-knockout-hek-293t-cell-line-ab266413'>ab266413</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

All lanes:

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

All lanes:

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution

All lanes:

293 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)

All lanes:

Western blot - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution

Lane 1:

Caco 2 cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false