Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (AB187363)

Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124954 at a dilution of 1 in 120 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (AB187363)

Immunofluorescence staining of HeLa cells with purified ab124954 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124954 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (AB187363)

Immunohistochemical staining of paraffin embedded human testis with purified ab124954 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (AB187363)

Unpurified ab124954, at 1/100 dilution, staining TXNRD1 in HeLa cells by Immunofluorescence.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

• IP

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Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] - BSA and Azide free (AB187363)

ab124954 (purified) at 1/40 immunoprecipitating TXNRD1 in 10 μg Jurkat (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124954).

All lanes:

Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] (<a href='/en-us/products/primary-antibodies/txnrd1-antibody-epncir129-ab124954'>ab124954</a>)

Predicted band size: 71 kDa

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