AB249425

Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TXNRD1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

GRIM12, KDRF, TXNRD1, TR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Peroxidase TXNRD1, Thioredoxin reductase TR1, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.

ab167411 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.

ab167411 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TXNRD1 with ab167411 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.

ab167411 showing +ve staining in Human normal liver.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling TXNRD1 with ab167411 at 1/100 dilution (red) or with a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells labeling TXNRD1 with ab167411 at 1/250 dilution. DAPI nuclear staining (blue).

Unknown

Western blot - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

This data was developed using ab167411, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TXNRD1 antibody [EPR10205] (<a href='/en-us/products/primary-antibodies/txnrd1-antibody-epr10205-ab167411'>ab167411</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 71 kDa

false

Unconjugated

Anti-TXNRD1 antibody [EPR10205]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10205

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249425 is the carrier-free version of ab167411.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin reductase 1 (TXNRD1) also known as TRXR1 is a selenoprotein that plays an important role in maintaining cellular redox balance by catalyzing the reduction of thioredoxin (TRX). It possesses a molecular mass of approximately 55 kDa. TXNRD1 can be found in many tissues displaying a stronger presence in the liver kidney and pancreas indicating its significance in these organs. The enzyme contains a selenocysteine residue at its active site which is essential for its enzymatic activity.

Biological function summary

TXNRD1 functions as an integral part of the thioredoxin system which includes thioredoxin and NADPH. It maintains the redox homeostasis in cells by regenerating oxidized thioredoxin. This action allows thioredoxin to in turn reduce disulfide bonds in various substrate proteins. Furthermore TXNRD1 participates in regulating cellular processes such as DNA synthesis and repair signal transduction and antioxidant defense. It does not work alone but collaborates with various redox-active components to achieve its roles.

Pathways

TXNRD1 participates in the antioxidant defense and redox signaling pathways. Within the antioxidant defense pathway TXNRD1 along with thioredoxin provides resistance to oxidative stress. In the redox signaling pathway the protein interacts with NF-κB signaling processes impacting cell inflammation and apoptosis. These pathways are vital for cellular health and are connected through thioredoxin emphasizing the interconnected nature of TXNRD1-related processes.

TXNRD1 has associations with cancer and neurodegenerative diseases. Elevated levels of TXNRD1 have been observed in various cancers including lung and hepatocellular carcinoma suggesting a role in tumor growth and survival through redox regulation. Additionally TXNRD1 is linked to neurodegenerative diseases such as Alzheimer's where oxidative stress plays a contributory role. In these conditions TXNRD1 interacts closely with other redox proteins like glutathione reductase highlighting its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form (PubMed : 8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed : 10849437).. Isoform 1. Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.. Isoform 4. Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.. Isoform 5. Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed : 15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed : 9774665).

See full target information TXNRD1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

Western blot - Anti-TXNRD1 antibody [EPR10205] - BSA and Azide free (AB249425)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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