AB180493

Anti-TXNRD2 antibody [EPR12480]

RabMAb
Recombinant
KO Validated
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(9 Publications)

Rabbit Recombinant Monoclonal TXNRD2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications.

View Alternative Names

KIAA1652, TRXR2, TXNRD2, Selenoprotein Z, TR-beta, Thioredoxin reductase TR3, SelZ

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] (AB180493)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] (AB180493)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TXNRD2 with ab180493 at 1/500 dilution followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-TXNRD2 antibody [EPR12480] (AB180493)

All lanes:

Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493) at 1/1000 dilution

Lane 1:

LnCap cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

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Lab

Western blot - Anti-TXNRD2 antibody [EPR12480] (AB180493)

Lanes 1-4 : Merged signal (red and green). Green - ab180493 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.

ab180493 Anti-TXNRD2 antibody [EPR12480] was shown to specifically react with TXNRD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267267 (knockout cell lysate ab258259) was used. Wild-type and TXNRD2 knockout samples were subjected to SDS-PAGE. ab180493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TXNRD2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TXNRD2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-txnrd2-knockout-hek-293t-cell-line-ab267267'>ab267267</a>)

Lane 3:

LNCap cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Supplier Data

Western blot - Anti-TXNRD2 antibody [EPR12480] (AB180493)

All lanes:

Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493) at 1/5000 dilution

Lane 1:

Fetal liver tissue lysate at 20 µg

Lane 2:

Fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

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Carrier free

Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12480

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin reductase 2 (TXNRD2) also known as TRXR2 is an important enzyme in the thioredoxin system. It has a mass of approximately 57 kDa and resides mainly in the mitochondria. TXNRD2 acts as a homodimer and contains a selenocysteine residue at its active site which is essential for its enzymatic activity. TXNRD2 is expressed ubiquitously but shows higher levels in tissues with high metabolic activity such as the heart muscle and brain. Its primary mechanical role involves the reduction of thioredoxin which in turn assists in reducing other proteins by cysteine thiol-disulfide exchange.

Biological function summary

TXNRD2 plays an important role in maintaining cellular redox balance and combating oxidative stress. It is part of the thioredoxin system which together with thioredoxin and thioredoxin peroxidase constitutes a defense against reactive oxygen species (ROS). TXNRD2 helps facilitate DNA synthesis and repair protein folding and the regulation of apoptosis. As a member of this protective complex TXNRD2 contributes to safeguarding cellular function under various stress conditions by modulating the reduction capacity of cells.

Pathways

TXNRD2 is importantly involved in the antioxidant defense and mitochondrial function pathways. In the antioxidant defense pathway it works in conjunction with the glutathione system to reduce ROS and repair oxidative damage. Additionally in mitochondrial function TXNRD2 collaborates with peroxiredoxins and other mitochondrial antioxidant systems to ensure the integrity of the electron transport chain and prevent oxidative damage within the mitochondria. TXNRD2 shares functional relations with proteins like thioredoxin 1 and peroxiredoxin 3 in these pathways.

TXNRD2 has significant implications in cardiovascular diseases and cancer. Mutations or dysregulation of TXNRD2 can lead to increased oxidative stress and myocardial dysfunction contributing to heart failure. Similarly altered TXNRD2 expression has been associated with tumor progression due to its role in managing cellular apoptosis and antioxidant defense allowing cancer cells to survive in oxidative environments. TXNRD2’s interactions with the protein NADPH oxidase highlight its involvement in these conditions where irregular ROS production exacerbates disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the control of reactive oxygen species levels and the regulation of mitochondrial redox homeostasis (PubMed : 24601690). Maintains thioredoxin in a reduced state. May play a role in redox-regulated cell signaling.

See full target information TXNRD2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 82:3486-3498 PubMed35916672

2022

Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate.

Applications

Unspecified application

Species

Unspecified reactive species

Connor S R Jankowski,Joshua D Rabinowitz

Genes 13: PubMed35327975

2022

Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Evke,Qishan Lin,Juan Andres Melendez,Thomas John Begley

Redox biology 37:101746 PubMed33059313

2020

Copper interferes with selenoprotein synthesis and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Schwarz,Kristina Lossow,Katja Schirl,Julian Hackler,Kostja Renko,Johannes Florian Kopp,Tanja Schwerdtle,Lutz Schomburg,Anna Patricia Kipp

Epigenetics 15:1121-1138 PubMed32303148

2020

The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Leonardi,Nataliia Kovalchuk,Lei Yin,Lauren Endres,Sara Evke,Steven Nevins,Samuel Martin,Peter C Dedon,J Andres Melendez,Laura Van Winkle,Qing-Yu Zhang,Xinxin Ding,Thomas J Begley

Redox biology 28:101345 PubMed31669973

2019

Metformin protects against PM-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2.

Applications

Unspecified application

Species

Unspecified reactive species

Junling Gao,Juntao Yuan,Qiao'e Wang,Tong Lei,Xiyue Shen,Bingqing Cui,Fang Zhang,Wenjun Ding,Zhongbing Lu

Toxicology research 8:641-653 PubMed31588341

2019

TrxR2 overexpression alleviates inflammation-mediated neuronal death reducing the oxidative stress and activating the Akt-Parkin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbao Gao,Yunjun Li,Wende Li,Haijiang Wang

The Journal of physiology 597:869-887 PubMed30556208

2018

Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness.

Applications

Unspecified application

Species

Unspecified reactive species

Michael D Tarpey,Ana P Valencia,Kathryn C Jackson,Adam J Amorese,Nicholas P Balestrieri,Randall H Renegar,Stephen J P Pratt,Terence E Ryan,Joseph M McClung,Richard M Lovering,Espen E Spangenburg

Free radical biology & medicine 121:202-214 PubMed29753072

2018

AMPKα2 deficiency exacerbates long-term PM exposure-induced lung injury and cardiac dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Wang,Xiyue Shen,Guoxiong Tian,Xili Shi,Wei Huang,Yongguang Wu,Lei Sun,Can Peng,Shasha Liu,Ying Huang,Xiaoyu Chen,Fang Zhang,Yingjie Chen,Wenjun Ding,Zhongbing Lu

Free radical biology & medicine 112:253-266 PubMed28774815

2017

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Blecha,Silvia Magalhaes Novais,Katerina Rohlenova,Eliska Novotna,Sandra Lettlova,Sabine Schmitt,Hans Zischka,Jiri Neuzil,Jakub Rohlena

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TXNRD2 antibody [EPR12480]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] (AB180493)

Western blot - Anti-TXNRD2 antibody [EPR12480] (AB180493)

Western blot - Anti-TXNRD2 antibody [EPR12480] (AB180493)

Western blot - Anti-TXNRD2 antibody [EPR12480] (AB180493)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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