AB250196

Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal TXNRD2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1652, TRXR2, TXNRD2, Selenoprotein Z, TR-beta, Thioredoxin reductase TR3, SelZ

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

This data was developed using ab180493, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TXNRD2 with ab180493 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

This data was developed using ab180493, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TXNRD2 antibody [EPR12480] (<a href='/en-us/products/primary-antibodies/txnrd2-antibody-epr12480-ab180493'>ab180493</a>) at 1/1000 dilution

Lane 1:

LnCap cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Lab

Western blot - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

This data was developed using ab180493, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab180493 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.

ab180493 Anti-TXNRD2 antibody [EPR12480] was shown to specifically react with TXNRD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267267 (knockout cell lysate ab258259) was used. Wild-type and TXNRD2 knockout samples were subjected to SDS-PAGE. ab180493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TXNRD2 antibody [EPR12480] (<a href='/en-us/products/primary-antibodies/txnrd2-antibody-epr12480-ab180493'>ab180493</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

TXNRD2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human TXNRD2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-txnrd2-knockout-hek-293t-cell-line-ab267267'>ab267267</a>)

Lane 3:

LNCap cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Supplier Data

Western blot - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

This data was developed using ab180493, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TXNRD2 antibody [EPR12480] (<a href='/en-us/products/primary-antibodies/txnrd2-antibody-epr12480-ab180493'>ab180493</a>) at 1/5000 dilution

Lane 1:

Fetal liver tissue lysate at 20 µg

Lane 2:

Fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Unconjugated

Anti-TXNRD2 antibody [EPR12480]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12480

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250196 is the carrier-free version of ab180493.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin reductase 2 (TXNRD2) also known as TRXR2 is an important enzyme in the thioredoxin system. It has a mass of approximately 57 kDa and resides mainly in the mitochondria. TXNRD2 acts as a homodimer and contains a selenocysteine residue at its active site which is essential for its enzymatic activity. TXNRD2 is expressed ubiquitously but shows higher levels in tissues with high metabolic activity such as the heart muscle and brain. Its primary mechanical role involves the reduction of thioredoxin which in turn assists in reducing other proteins by cysteine thiol-disulfide exchange.

Biological function summary

TXNRD2 plays an important role in maintaining cellular redox balance and combating oxidative stress. It is part of the thioredoxin system which together with thioredoxin and thioredoxin peroxidase constitutes a defense against reactive oxygen species (ROS). TXNRD2 helps facilitate DNA synthesis and repair protein folding and the regulation of apoptosis. As a member of this protective complex TXNRD2 contributes to safeguarding cellular function under various stress conditions by modulating the reduction capacity of cells.

Pathways

TXNRD2 is importantly involved in the antioxidant defense and mitochondrial function pathways. In the antioxidant defense pathway it works in conjunction with the glutathione system to reduce ROS and repair oxidative damage. Additionally in mitochondrial function TXNRD2 collaborates with peroxiredoxins and other mitochondrial antioxidant systems to ensure the integrity of the electron transport chain and prevent oxidative damage within the mitochondria. TXNRD2 shares functional relations with proteins like thioredoxin 1 and peroxiredoxin 3 in these pathways.

TXNRD2 has significant implications in cardiovascular diseases and cancer. Mutations or dysregulation of TXNRD2 can lead to increased oxidative stress and myocardial dysfunction contributing to heart failure. Similarly altered TXNRD2 expression has been associated with tumor progression due to its role in managing cellular apoptosis and antioxidant defense allowing cancer cells to survive in oxidative environments. TXNRD2’s interactions with the protein NADPH oxidase highlight its involvement in these conditions where irregular ROS production exacerbates disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the control of reactive oxygen species levels and the regulation of mitochondrial redox homeostasis (PubMed : 24601690). Maintains thioredoxin in a reduced state. May play a role in redox-regulated cell signaling.

See full target information TXNRD2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International heart journal 64:60-70 PubMed36725079

2023

Thioredoxin Reductase 2 Synergizes with Cytochrome c, Somatic to Alleviate Doxorubicin-Induced Oxidative Stress in Cardiomyocytes and Mouse Myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Chuyun Li,Hengli Lai,Xinyong Cai,Yuanyuan Liu,Lang Hong,Xiao Huang,Liang Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

Western blot - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

Western blot - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

Western blot - Anti-TXNRD2 antibody [EPR12480] - BSA and Azide free (AB250196)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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