Anti-TYK2 antibody

• WB

Lab

Western blot - Anti-TYK2 antibody (AB223733)

False colour image of Western blot : Anti-TYK2 antibody staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223733 was shown to bind specifically to TYK2. A band was observed at 133 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TYK2 knockout cell line ab266730 (knockout cell lysate ab258743). To generate this image, wild-type and TYK2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-TYK2 antibody (ab223733) at 0.04 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

TYK2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human TYK2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tyk2-knockout-hek-293t-cell-line-ab266730'>ab266730</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 134 kDa

Observed band size: 133 kDa

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TYK2 antibody (AB223733)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TYK2 (green) using ab223733 at 4 μg/ml in ICC/IF.

• WB

Supplier Data

Western blot - Anti-TYK2 antibody (AB223733)

All lanes:

Western blot - Anti-TYK2 antibody (ab223733) at 1/100 dilution

All lanes:

PC-3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 134 kDa

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