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Rabbit Polyclonal TYK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TYK2 aa 200-400.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human TYK2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link P29597
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Target data

Function

Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.

Alternative names

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Rabbit Polyclonal TYK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TYK2 aa 200-400.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human TYK2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link P29597
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.

Activity summary

Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.

Pathways

TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.

Associated diseases and disorders

Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.

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3 product images

  • Western blot - Anti-TYK2 antibody (ab223733), expandable thumbnail

    Western blot - Anti-TYK2 antibody (ab223733)

    False colour image of Western blot: Anti-TYK2 antibody staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223733 was shown to bind specifically to TYK2. A band was observed at 133 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TYK2 knockout cell line ab266730 (knockout cell lysate ab258743). To generate this image, wild-type and TYK2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-TYK2 antibody (AB223733) at 0.04 µg/mL

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: TYK2 knockout HEK-293T cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 134 kDa

    Observed band size: 133 kDa

  • Western blot - Anti-TYK2 antibody (ab223733), expandable thumbnail

    Western blot - Anti-TYK2 antibody (ab223733)

    All lanes: Western blot - Anti-TYK2 antibody (AB223733) at 1/100 dilution

    All lanes: PC-3 (human prostate adenocarcinoma cell line) cell lysate

    Predicted band size: 134 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TYK2 antibody (ab223733), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TYK2 antibody (ab223733)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TYK2 (green) using ab223733 at 4 μg/ml in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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