Anti-TYK2 antibody (ab223733) is a rabbit polyclonal antibody that is used to detect TYK2 in Western Blot, ICC/IF. Suitable for Human samples.
- Specificity confirmed with TYK2 knockout cell line validation
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- Clinical cancer research : an official journal of the American Association for Cancer Research 29:1592-16042023MEK Inhibition Synergizes with TYK2 Inhibitors in NF1-Associated Malignant Peripheral Nerve Sheath Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDana C Borcherding,Neha V Amin,Kevin He,Xiaochun Zhang,Yang Lyu,Carina Dehner,Himanshi Bhatia,Angad Gothra,Layla Daud,Peter Ruminski,Christine A Pratilas,Kai Pollard,Taylor Sundby,Brigitte C Widemann,Angela C HirbePubMed 36799629
- Cell reports 41:1115612022OTUB2 exerts tumor-suppressive roles via STAT1-mediated CALML3 activation and increased phosphatidylserine synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWan Chang,Qingyu Luo,Xiaowei Wu,Yabing Nan,Pengfei Zhao,Lingqiang Zhang,Aiping Luo,Wenjie Jiao,Qiong Zhu,Yesheng Fu,Zhihua LiuPubMed 36288705
- Journal of virology 95:e00266212021SARS-CoV-2 Nonstructural Protein 1 Inhibits the Interferon Response by Causing Depletion of Key Host Signaling Factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnil Kumar,Ray Ishida,Tania Strilets,Jamie Cole,Joaquin Lopez-Orozco,Nawell Fayad,Alberto Felix-Lopez,Mohamed Elaish,Danyel Evseev,Katharine E Magor,Lara K Mahal,Les P Nagata,David H Evans,Tom C HobmanPubMed 34110264
- Journal of experimental & clinical cancer research : CR 40:1052021Cirsiliol targets tyrosine kinase 2 to inhibit esophageal squamous cell carcinoma growth in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuechao Jia,Chuntian Huang,Yamei Hu,Qiong Wu,Fangfang Liu,Wenna Nie,Hanyong Chen,Xiang Li,Zigang Dong,Kangdong LiuPubMed 33731185
- Cellular physiology and biochemistry : internation 50:2188-22022018Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDexin Yin et. al.PubMed 30415256
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TYK2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link P29597
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity (PubMed:10542297, PubMed:10995743, PubMed:7657660, PubMed:7813427, PubMed:8232552). Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling (PubMed:10542297). Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed:10542297, PubMed:7638186). The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2 (PubMed:10542297, PubMed:25762719, PubMed:7526154, PubMed:7813427). In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members (PubMed:7526154, PubMed:7657660). In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes (PubMed:10542297, PubMed:25762719, PubMed:7657660). Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed:29162862).
Alternative names
Non-receptor tyrosine-protein kinase TYK2, TYK2
Recommended products
Anti-TYK2 antibody (ab223733) is a rabbit polyclonal antibody that is used to detect TYK2 in Western Blot, ICC/IF. Suitable for Human samples.
- Specificity confirmed with TYK2 knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TYK2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link P29597
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TYK2 antibody (ab223733) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.
What is the molecular weight of TYK2?
Anti-TYK2 (ab223733) specifically detects a band for TYK2 (UniProt: P29597) at a molecular weight of 134kDa.
Recommended positive controls
WB: PC-3 cell lysate. HEK-293, MCF-7, Jurkat cells.ICC/IF: U-2 OS cells.
Specificity confirmed
The specificity of Anti-TYK2 antibody (ab223733) has been confirmed by Western blot testing in TYK2 Knockout HEK293T cell line, Human TYK2 knockout HEK-293T cell line ab266730.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.
- Biological function summary
TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.
- Pathways
TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.
- Associated diseases and disorders
Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TYK2 antibody (ab223733)
False colour image of Western blot: Anti-TYK2 antibody staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223733 was shown to bind specifically to TYK2. A band was observed at 133 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TYK2 knockout cell line Human TYK2 knockout HEK-293T cell line ab266730 (knockout cell lysate Human TYK2 knockout HEK-293T cell lysate ab258743). To generate this image, wild-type and TYK2 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-TYK2 antibody (ab223733) at 0.04 µg/mL
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: TYK2 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human TYK2 knockout HEK-293T cell line (Human TYK2 knockout HEK-293T cell line ab266730)
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 134 kDa
Observed band size: 133 kDa
Western blot - Anti-TYK2 antibody (ab223733)
All lanes: Western blot - Anti-TYK2 antibody (ab223733) at 1/100 dilution
All lanes: PC-3 (human prostate adenocarcinoma cell line) cell lysate
Predicted band size: 134 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TYK2 antibody (ab223733)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TYK2 (green) using ab223733 at 4 μg/ml in ICC/IF.
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