JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314878

Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TYK2 phospho Y1054 + Y1055 antibody. Suitable for WB, IP, Dot and reacts with Human, Synthetic peptide samples.

View Alternative Names

Non-receptor tyrosine-protein kinase TYK2, TYK2

3 Images
Immunoprecipitation - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (AB314878)
  • IP

Supplier Data

Immunoprecipitation - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (AB314878)

TYK2 (phospho Y1054 + Y1055) was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate with ab314878 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314878 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate Lane 2 : ab314878 IP in U266B1(human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314878 in U266B1 starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (ab314878) at 1/30 dilution

All lanes:

U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Western blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (AB314878)
  • WB

Supplier Data

Western blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (AB314878)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-TYK2 antibody - Total protein control (ab303500) staining at 1/1000 dilution

All lanes:

Western blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (ab314878) at 1/1000 dilution

Lane 1:

Untreated U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours whole cell lysate (untreated membrane) at 20 µg

Lane 2:

U266B1 starved for 16 hours, then treated with 50ng/ml IFN alpha and 10μM MG-132 for 15 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

U266B1 starved for 16 hours, then treated with 50ng/ml IFN alpha and 10μM MG-132 for 15 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 135 kDa,37 kDa,140 kDa

false

Exposure time: 180s

Dot Blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (AB314878)
  • Dot

Supplier Data

Dot Blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (AB314878)

Dot blot analysis of TYK2 (phospho Y1054 + Y1055) using ab314878 at 1 : 1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : TYK2 (phospho Tyr1054+Tyr1055) peptide a Lane 2 : TYK2 (phospho Tyr1054+Tyr1055) peptide b Lane 3 : TYK2 (phospho Tyr1054) peptide c Lane 4 : TYK2 (phospho Tyr1055) peptide d Lane 5 : TYK2 non-phospho peptide e Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Carrier free

    Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28591-25

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB316897

Human TYK2 ELISA Kit

SimpleStep

Sandwich ELISA - Human TYK2 ELISA Kit (AB316897)

  • 0
  • 0
Primary Antibodies

AB138005

Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 (phospho Y1034 + Y1035) antibody [EPR1899(2)] (AB138005)

  • 5
  • 2
Primary Antibodies

AB32101

Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101)

  • 3
  • 4
Primary Antibodies

AB92506

Anti-STAT1 alpha antibody [EPYR2154]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Advanced Validation

Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (AB92506)

  • 4
  • 2
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.
Biological function summary

TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.

Pathways

TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.

Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity (PubMed : 10542297, PubMed : 10995743, PubMed : 7657660, PubMed : 7813427, PubMed : 8232552). Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling (PubMed : 10542297). Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed : 10542297, PubMed : 7638186). The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2 (PubMed : 10542297, PubMed : 25762719, PubMed : 7526154, PubMed : 7813427). In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members (PubMed : 7526154, PubMed : 7657660). In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes (PubMed : 10542297, PubMed : 25762719, PubMed : 7657660). Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed : 29162862).
See full target information TYK2 phospho Y1054 + Y1055
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com