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AB314879

Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free

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Rabbit Recombinant Monoclonal TYK2 phospho Y1054 + Y1055 antibody. Carrier free. Suitable for WB, IP, Dot and reacts with Human, Synthetic peptide samples.

View Alternative Names

Non-receptor tyrosine-protein kinase TYK2, TYK2

3 Images
Immunoprecipitation - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free (AB314879)
  • IP

Supplier Data

Immunoprecipitation - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free (AB314879)

This data was developed using ab314878, the same antibody clone in a different buffer formulation. TYK2 (phospho Y1054 + Y1055) was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate with ab314878 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314878 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate 10 µg Lane 2 : ab314878 IP in U266B1(human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314878 in U266B1 starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (<a href='/en-us/products/primary-antibodies/tyk2-phospho-y1054-y1055-antibody-epr28591-25-ab314878'>ab314878</a>) at 1/30 dilution

All lanes:

U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours, then treated with 50ng/ml IFN alpha for 15 minutes whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Western blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free (AB314879)
  • WB

Supplier Data

Western blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free (AB314879)

This data was developed usingab314878, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-TYK2 antibody - Total protein control (ab303500) staining at 1/1000 dilution

All lanes:

Western blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] (<a href='/en-us/products/primary-antibodies/tyk2-phospho-y1054-y1055-antibody-epr28591-25-ab314878'>ab314878</a>) at 1/1000 dilution

Lane 1:

Untreated U266B1 (human multiple myeloma B lymphocyte) starved for 16 hours whole cell lysate (untreated membrane) at 20 µg

Lane 2:

U266B1 starved for 16 hours, then treated with 50ng/ml IFN alpha and 10μM MG-132 for 15 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

U266B1 starved for 16 hours, then treated with 50ng/ml IFN alpha and 10μM MG-132 for 15 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 135 kDa,37 kDa,140 kDa

false

Exposure time: 180s

Dot Blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free (AB314879)
  • Dot

Supplier Data

Dot Blot - Anti-TYK2 (phospho Y1054 + Y1055) antibody [EPR28591-25] - BSA and Azide free (AB314879)

Dot blot analysis of TYK2 (phospho Y1054 + Y1055) using ab314878 at 1 : 1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : TYK2 (phospho Tyr1054+Tyr1055) peptide a Lane 2 : TYK2 (phospho Tyr1054+Tyr1055) peptide b Lane 3 : TYK2 (phospho Tyr1054) peptide c Lane 4 : TYK2 (phospho Tyr1055) peptide d Lane 5 : TYK2 non-phospho peptide e Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28591-25

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314879 is the carrier-free version of ab314878.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine kinase 2 (TYK2) also known as JAK1B is a non-receptor tyrosine kinase that weighs approximately 134 kDa. It belongs to the Janus kinase (JAK) family which includes JAK1 JAK2 and JAK3. TYK2 is vital in signal transduction for several cytokine receptors. The expression of TYK2 occurs broadly in different tissues including immune system cells. It plays an important mechanical role in transferring signals from cell surface receptors to intracellular signaling pathways which modulates various physiological processes.
Biological function summary

TYK2 mediates intracellular signaling that affects both innate and adaptive immune responses. It associates with cytokine receptors forming functional complexes that are critical for initiating JAK-STAT signaling. This type of signaling is responsible for regulating gene expression after cytokine binding. TYK2 is known for interacting with Type I interferons and interleukin-10 family cytokines indicating its importance in immune regulation.

Pathways

TYK2 acts within the JAK-STAT pathway a fundamental signaling cascade triggered by various cytokines and growth factors. The JAK-STAT pathway includes key partners such as STAT1 STAT2 and STAT3 proteins which are phosphorylated by TYK2 to propagate signal transduction. Additionally TYK2 is involved in the 'intercept blocking buffer' mechanism that adjusts signal strength thereby ensuring appropriate cellular responses to external stimuli.

Altered TYK2 activity has associations with immune-related conditions like psoriasis and systemic lupus erythematosus. Dysregulation of TYK2 can lead to improper immune signaling which contributes to these autoimmune conditions. Furthermore TYK2 interacts with other Janus kinase family members including JAK1 and JAK3 which can influence its role in the pathogenesis of these diseases potentially offering targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity (PubMed : 10542297, PubMed : 10995743, PubMed : 7657660, PubMed : 7813427, PubMed : 8232552). Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling (PubMed : 10542297). Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6 (PubMed : 10542297, PubMed : 7638186). The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2 (PubMed : 10542297, PubMed : 25762719, PubMed : 7526154, PubMed : 7813427). In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members (PubMed : 7526154, PubMed : 7657660). In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes (PubMed : 10542297, PubMed : 25762719, PubMed : 7657660). Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling (PubMed : 29162862).
See full target information TYK2 phospho Y1054 + Y1055
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Product promise

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