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Rabbit Recombinant Monoclonal TYRO3 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (AB109231), expandable thumbnail
  • Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (AB109231), expandable thumbnail
  • Western blot - Anti-TYRO3 antibody [EPR4308] (AB109231), expandable thumbnail
  • Western blot - Anti-TYRO3 antibody [EPR4308] (AB109231), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (AB109231), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Tested
Not recommended
Tested
Mouse
Expected
Expected
Not recommended
Tested
Rat
Expected
Expected
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

For unpurified use at 1/100 - 1/250.

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

For unpurified use at 1/10 - 1/100.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

Alternative names

BYK, DTK, RSE, SKY, TIF, TYRO3, Tyrosine-protein kinase receptor TYRO3, Tyrosine-protein kinase BYK, Tyrosine-protein kinase DTK, Tyrosine-protein kinase RSE, Tyrosine-protein kinase SKY, Tyrosine-protein kinase TIF

Recommended products

Rabbit Recombinant Monoclonal TYRO3 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4308
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TYRO3 also known as Tyrosine-protein kinase receptor TYRO3 is a membrane protein involved in various cellular processes. The protein has a molecular mass of approximately 104 kDa. TYRO3 expresses in a range of tissues with high levels noted in the central nervous system reproductive system and immune cells. The protein is part of the TAM family of receptor tyrosine kinases which includes AXL and MERTK and it participates in binding to specific ligands for signal transmission.

Biological function summary

TYRO3 influences multiple cellular activities such as cell survival proliferation and immune regulation. It acts within a signaling complex that includes ligands like Gas6 and Protein S. TYRO3 plays roles in phagocytosis of apoptotic cells and regulation of inflammatory responses. The protein's signaling pathways support tissue homeostasis and modulate immune responses making it an important player in maintaining cellular balance.

Pathways

TYRO3 is engaged in the PI3K/AKT and MAPK pathways which facilitate cell cycle progression and survival. In these pathways it interacts with other proteins such as AXL MERTK and downstream effectors like AKT and ERK. These interactions ensure proper cellular functions with TYRO3-mediated pathways contributing to normal cellular homeostasis and protective responses against stress.

Associated diseases and disorders

Disruptions in TYRO3 function associate with cancer and autoimmune diseases. Its overexpression or mutations can lead to abnormal cell growth linking TYRO3 to certain cancers. The protein also intersects with immune-related disorders where an imbalance in TYRO3 signaling contributes to diseases like systemic lupus erythematosus. Proteins like AXL and MERTK closely related to TYRO3 often share these pathological associations emphasizing the interconnected nature of the TAM receptor family in disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (ab109231)

    ab109231 (purified) at 1:200 dilution (2μg) immunoprecipitating TYRO3 in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab109231 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109231 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:2000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Predicted band size: 97 kDa

  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)

    All lanes: Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution

    Lane 1: Rat hippocampus lysates at 20 µg

    Lane 2: Rat brain lysates at 20 µg

    Lane 3: Human fetal brain lysates at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 97 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)

    All lanes: Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution

    All lanes: Mouse hippocampus lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa

    Observed band size: 120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Immunohistochemical analysis of TYRO3 in paraffin embedded Human brain tissue, using ab109231 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231), expandable thumbnail

    Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)

    All lanes: Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution

    Lane 1: fetal brain lysates at 10 µg

    Lane 2: fetal hippocampus lysates at 10 µg

    Lane 3: MCF7 cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 97 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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