AB117877

Anti-TYRO3 antibody [OTI5B7]

Name: Anti-TYRO3 antibody [OTI5B7] (ab117877) | Abcam
Brand: Abcam Limited
SKU: AB117877
Price: 525 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal TYRO3 antibody. Suitable for IP, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TYRO3.

View Alternative Names

BYK, DTK, RSE, SKY, TIF, TYRO3, Tyrosine-protein kinase receptor TYRO3, Tyrosine-protein kinase BYK, Tyrosine-protein kinase DTK, Tyrosine-protein kinase RSE, Tyrosine-protein kinase SKY, Tyrosine-protein kinase TIF

3 Images

Flow Cytometry (Intracellular) - Anti-TYRO3 antibody [OTI5B7] (AB117877)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TYRO3 antibody [OTI5B7] (AB117877)

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY TYRO3 (Red) or empty vector control plasmid (Blue) were immunostained with ab117877 at 1/100 dilution and then analyzed by Flow Cytometry (Intracellular).

Supplier Data

Immunoprecipitation - Anti-TYRO3 antibody [OTI5B7] (AB117877)

For each experiment, 500 μl of DDK tagged TYRO3 overexpression lysates (at 1 : 5 dilution with HEK-293T lysate), 2 μg of ab117877 and 20 μl (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Negative control: IP without adding ab117877

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Immunoprecipitation - Anti-TYRO3 antibody [OTI5B7] (ab117877) at 2 µg

Predicted band size: 15 kDa,161 kDa,35 kDa,46 kDa,52 kDa,53 kDa,54 kDa,61 kDa,64 kDa,67 kDa,87 kDa,97 kDa

Observed band size: 16 kDa,36 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-TYRO3 antibody [OTI5B7] (AB117877)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TYRO3 antibody [OTI5B7] (AB117877)

pCMV6-ENTRY TYRO3 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for TYRO3 using ab117877 at a 1/100 dilution in ICC/IF.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI5B7

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TYRO3.

Q06418

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody." }, "Transfected cell lysate - Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "4 µg/mL", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The clone number has been updated from 5B7 to OTI5B7, both clone numbers name the same clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TYRO3 also known as Tyrosine-protein kinase receptor TYRO3 is a membrane protein involved in various cellular processes. The protein has a molecular mass of approximately 104 kDa. TYRO3 expresses in a range of tissues with high levels noted in the central nervous system reproductive system and immune cells. The protein is part of the TAM family of receptor tyrosine kinases which includes AXL and MERTK and it participates in binding to specific ligands for signal transmission.

Biological function summary

TYRO3 influences multiple cellular activities such as cell survival proliferation and immune regulation. It acts within a signaling complex that includes ligands like Gas6 and Protein S. TYRO3 plays roles in phagocytosis of apoptotic cells and regulation of inflammatory responses. The protein's signaling pathways support tissue homeostasis and modulate immune responses making it an important player in maintaining cellular balance.

Pathways

TYRO3 is engaged in the PI3K/AKT and MAPK pathways which facilitate cell cycle progression and survival. In these pathways it interacts with other proteins such as AXL MERTK and downstream effectors like AKT and ERK. These interactions ensure proper cellular functions with TYRO3-mediated pathways contributing to normal cellular homeostasis and protective responses against stress.

Disruptions in TYRO3 function associate with cancer and autoimmune diseases. Its overexpression or mutations can lead to abnormal cell growth linking TYRO3 to certain cancers. The protein also intersects with immune-related disorders where an imbalance in TYRO3 signaling contributes to diseases like systemic lupus erythematosus. Proteins like AXL and MERTK closely related to TYRO3 often share these pathological associations emphasizing the interconnected nature of the TAM receptor family in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

See full target information TYRO3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 12:1164-1170 PubMed27446413

2016

Lidocaine inhibits the invasion and migration of TRPV6-expressing cancer cells by TRPV6 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Jiang,Hui Gou,Jiang Zhu,Si Tian,Lehua Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TYRO3 antibody [OTI5B7]

Flow Cytometry (Intracellular) - Anti-TYRO3 antibody [OTI5B7] (AB117877)

Immunoprecipitation - Anti-TYRO3 antibody [OTI5B7] (AB117877)

Immunocytochemistry/ Immunofluorescence - Anti-TYRO3 antibody [OTI5B7] (AB117877)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise