Anti-Tyrosinase antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody (AB180753)

Immunofluorescence staining of human skin cancer stained for Tyrosinase with ab180753 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody (AB180753)

Immunofluorescence staining of mouse skin stained for Tyrosinase with ab180753 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody (AB180753)

Paraffin-embedded rat heart tissue stained for Tyrosinase using ab180753 at 1/100 dilution in immunohistochemical analysis.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody (AB180753)

Immunofluorescence staining of rat skin stained for Tyrosinase with ab180753 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

• WB

Unknown

Western blot - Anti-Tyrosinase antibody (AB180753)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Tyrosinase antibody (ab180753) at 1/1000 dilution

Lane 1:

Mouse craniofacial tissue lysate at 25 µg

Lane 2:

Mouse liver tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 60 kDa

false

• WB

CiteAb

Western blot - Anti-Tyrosinase antibody (AB180753)

Tyrosinase western blot using anti-Tyrosinase antibody ab180753. Publication image and figure legend from Challa, A. K., Boitet, E. R., et al., 2016, PLoS One, PubMed 27224051.

ab180753 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab180753 please see the product overview.

Comparison of dhoosara and chandana with the albino and black/wild type mice.(A) Dorsal view showing the coat color of albino, chandana, dhoosara, and black animals. Increasing levels of pigmentation can be seen in the hind limbs and tips of tails as well. (B) Frontal view of the face with increasing pigmentation in the nose region. Differences in the eye color can be noted. (C) Brightfield images of RPE wholemounts from Albino, chandana, dhoosara and Black mice. (D) Brightfield images of retinal cryosections from Albino, chandana, dhoosara and black mice. The RPE layer is the only layer within the eyeball that contains pigment. Scale bar = 50 μm. (E) Mean gray values (quantified in ImageJ) obtained from the RPE wholemounts reflecting relative intensity of RPE cell pigmentation. Results are presented in a bar chart with standard error of mean used for error bars. All groups are significantly different from each other (p<0.05, t-test). (F) Western Blot of tyrosinase protein isolated from skin of albino (alb), chandana (cha), dhoosara (dho), and black animals (arrow points to the 60 kDa tyrosinase band); 50 kDa tubulin protein (lower panel) is used as a control.

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