Anti-Tyrosine Hydroxylase antibody [4H2] - Neuronal Marker

• IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody [4H2] - Neuronal Marker (AB315207)

Immunofluorescent analysis of rat brain section stained with ab315207, dilution 1 : 1,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. The ab315207 antibody stains TH expressing neuronal processes, which are particularly numerous in the striatum, at the right of the image.

• WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody [4H2] - Neuronal Marker (AB315207)

Western blot analysis of tissue and cell lysates using ab315207, dilution 1 : 5,000 in green : [1] protein standard (red), [2] rat brain caudate/putmen and [3] PC12 cells. The strong band at about 58kDa corresponds to TH protein. The weak band at about 80kDa in the rat brain homogenate is likely an aggregated form of TH, which does not affect the specific cell and process staining of this antibody.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [4H2] - Neuronal Marker (ab315207) at 1/5000 dilution

Lane 2:

Rat brain caudate/putmen cell lysate

Lane 3:

PC12 cell lysate

Observed band size: 58 kDa

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