Rabbit Polyclonal Tyrosine Hydroxylase antibody. Suitable for WB, IHC-FoFr, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TH aa 150-500.
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- Oxidative medicine and cellular longevity 2022:76713242022miR-494-3p Promotes Erastin-Induced Ferroptosis by Targeting REST to Activate the Interplay between SP1 and ACSL4 in Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianjun Ma,Xiaohuan Li,Yongyan Fan,Dawei Yang,Qi Gu,Dongsheng Li,Siyuan Chen,Shaopu Wu,Jinhua Zheng,Hongqi Yang,Xue LiPubMed 35936219
- Frontiers in molecular neuroscience 14:6974402021Glucagon-Like Peptide-1 Receptor Agonist Ameliorates 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP) Neurotoxicity Through Enhancing Mitophagy Flux and Reducing α-Synuclein and Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsu-Kung Lin,Kai-Jung Lin,Hung-Yu Lin,Kai-Lieh Lin,Min-Yu Lan,Pei-Wen Wang,Tzu-Jou Wang,Feng-Sheng Wang,Po-Chin Tsai,Chia-Wei Liou,Jiin-Haur ChuangPubMed 34305527
- Frontiers in neuroanatomy 13:732019An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWonn S Pyon et. al.PubMed 31379520
- Journal of vascular research 54:259-2712017Fetal Sheep Mesenteric Resistance Arteries: Functional and Structural Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia J Müller,Matthias Schwab,Charles R Rosenfeld,Iwa Antonow-Schlorke,Peter W Nathanielsz,Florian Rakers,Harald Schubert,Otto W Witte,Sven RupprechtPubMed 28810262
- The international journal of neuropsychopharmacolo 17:1647-582014Long-lasting alterations in 5-HT2A receptor after a binge regimen of methamphetamine in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Yi Chiu et. al.PubMed 24763081
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TH aa 150-500. The exact immunogen used to generate this antibody is proprietary information. Database link P07101
Reactivity data
Select an application| WB | IHC-FoFr | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Tested | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays an important role in the physiology of adrenergic neurons (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).
Alternative names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TH, TYH
Recommended products
Rabbit Polyclonal Tyrosine Hydroxylase antibody. Suitable for WB, IHC-FoFr, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TH aa 150-500.
Alternative names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TH, TYH
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TH aa 150-500. The exact immunogen used to generate this antibody is proprietary information. Database link P07101
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
- Biological function summary
Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.
- Pathways
Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.
- Associated diseases and disorders
Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
Immunohistochemical analysis of paraffin-embedded H661 xenograft labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
7.5% SDS-PAGE
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/1000 dilution
All lanes: PC-12 whole cell lysate at 30 µg
Predicted band size: 58 kDa, 61 kDa, 71 kDa
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution
Lane 1: NT2D1 whole cell lysate at 30 µg
Lane 2: PC-3 whole cell lysate at 30 µg
Lane 3: U87-MG whole cell lysate at 30 µg
Lane 4: SK-N-SH whole cell lysate at 30 µg
Predicted band size: 58 kDa
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution
All lanes: Mouse brain lysate at 50 µg
Predicted band size: 58 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137721)
Immunofluorescent analysis of methanol-fixed SK-N-SH cells labelling Tyrosine Hydroxylase with ab137721 at 1/500 dilution (left panel) and co-stained with Hoechst 33343 (right panel).
SK-N-SH cells were fixed in -20°C 100% MeOH for 5 min.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)
Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
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