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Rabbit Recombinant Monoclonal Tyrosine Hydroxylase antibody. Suitable for ICC, IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TH aa 450-500.

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Images

Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080), expandable thumbnail
  • Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080), expandable thumbnail
  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (AB314080), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human TH aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link P07101

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIHC-PWB
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:15287903, PubMed:1680128, PubMed:17391063, PubMed:24753243, PubMed:34922205, PubMed:8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). Isoform 5. Lacks catalytic activity. Isoform 6. Lacks catalytic activity.

Alternative names

TYH, TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase

Recommended products

Rabbit Recombinant Monoclonal Tyrosine Hydroxylase antibody. Suitable for ICC, IHC-P, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human TH aa 450-500.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human TH aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link P07101
Clone number
BLR155J
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Associated diseases and disorders

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080)

    Samples: Whole cell lysate (10 µg) from HEK293T, SKN-SH, SK-N-BE(2), Jurkat, and SK-N-MC cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080) at 1/1000 dilution

    Lane 1: HEK293T Whole cell lysate at 10 µg

    Lane 2: SKN-SH Whole cell lysate at 10 µg

    Lane 3: SK-N-BE(2) Whole cell lysate at 10 µg

    Lane 4: Jurkat Whole cell lysate at 10 µg

    Lane 5: SK-N-MC Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080)

    Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, ND7/23, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080) at 1/1000 dilution

    Lane 1: NIH 3T3 Whole cell lysate at 50 µg

    Lane 2: CT26 Whole cell lysate at 50 µg

    Lane 3: ND7/23 Whole cell lysate at 50 µg

    Lane 4: TCMK-1 Whole cell lysate at 50 µg

    Lane 5: BW5147.3 Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080)

    Detection of human Tyrosine Hydroxylase in FFPE mouse olfactory bulb by immunohistochemistry. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080)

    Detection of human Tyrosine Hydroxylase in FFPE mouse brain by immunohistochemistry. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080), expandable thumbnail

    Immunocytochemistry - Anti-Tyrosine Hydroxylase antibody [BLR155J] - BSAfree (ab314080)

    Detection of human Tyrosine Hydroxylase in FFPE SK-NBE(2) cells by immunocytochemistry. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody [BLR155J] (A700-155CF). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

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