Chicken Recombinant Monoclonal Tyrosine Hydroxylase antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318307), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-ep1532y-chicken-igy-chimeric-bsa-and-azide-free-ab318307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432640.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318307), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-ep1532y-chicken-igy-chimeric-bsa-and-azide-free-ab318307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432639.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (AB318307)")
Key facts
- Isotype
IgY
- Host species
Chicken
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Mouse | Tested |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
15 products for Alternative Version
Target data
Function
Plays an important role in the physiology of adrenergic neurons (Probable). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed:30936473).
Alternative names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th
Recommended products
Chicken Recombinant Monoclonal Tyrosine Hydroxylase antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.
Alternative names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th
Key facts
- Isotype
IgY
- Host species
Chicken
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP1532Y
- Purification technique
Affinity purification Thiophilic Resin
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) – BSA and Azide Free ab318305 is the carrier-free version of Anti-Iba1 antibody [EPR16588] – Chicken IgY (Chimeric) ab318302.
This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker ab137869). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-ep1532y-chicken-igy-chimeric-bsa-and-azide-free-ab318307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432640.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307)
Immunofluorescence staining of tyrosine hydroxylase in a section of formalin-fixed paraffin-embedded mouse brain. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab318307 at 1/500 dilution, and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-ep1532y-chicken-igy-chimeric-bsa-and-azide-free-ab318307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img432639.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307)
Immunofluorescence staining of tyrosine hydroxylase in a section of formalin-fixed paraffin-embedded rat brain. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab318307 at 1/500 dilution, and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Downloads
Product protocols
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