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AB317796

Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free

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Mouse Recombinant Monoclonal Tyrosine Hydroxylase antibody. Carrier free. Suitable for IHC-P and reacts with Rat samples.

View Alternative Names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317796)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317796)

Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317796 at 1/500 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Unconjugated

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Mouse IgG1 (Chimeric)

  • Unconjugated

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • HRP

    HRP Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • Carrier free

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • 578 PE

    PE Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

  • Unconjugated

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric)

  • Carrier free

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free

  • Unconjugated

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Chicken IgY (Chimeric)

  • Carrier free

    Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EP1532Y

Isotype

IgG1

Carrier free

Yes

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab317796 is the carrier-free version of ab317786.

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab137869). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan but with lower specificity (PubMed : 10933781, PubMed : 11922614). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).
See full target information TH
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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