Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

• IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

ab137869 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21°C followed by incubation with the primary antibody for 2 hours at 1/800. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

Image courtesy of Carl Hobbs, Kings College London, U.K.

• IHC-P

AbReview39087****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

ab137869 staining Tyrosine Hydroxylase in rat brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21°C followed by incubation with the primary antibody for 2 hours at 1/1000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

Image courtesy of Carl Hobbs, Kings College London, U.K.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Immunohistochemical analysis of formalin fixed paraffin embedded human cortex labelling Tyrosine Hydroxylase with ab137869 at a concentration of 0.2 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab137869 anti-Tyrosine Hydroxylase [EP1532Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1 : 500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1 : 500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling Tyrosine Hydroxylase with Purified ab137869 at 1 : 100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labelling Tyrosine Hydroxylase antibody with ab137869 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with anti-rabbit HQ, anti-HQ HRP followed by ChromoMap DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab137869 anti-Tyrosine Hydroxylase antibody [EP1532Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• WB

AbReview51586****

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Blocked with 5% milk for 1 hour at 25°C.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 1/5000 dilution

Lane 1:

SH-SY5Y cell lysate - transduced with AAV vector expressing human TH at 20000 Cells

Lane 2:

SH-SY5Y cell lysate - non-infected at 20000 Cells

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

true

Exposure time: 1s

This image is courtesy of an Abreview submitted by Andre Antunes

• WB

Lab

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 0.1 µg/mL

All lanes:

Human adrenal gland lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID : 17157277).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (ab137869) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (<a href='/en-us/products/primary-antibodies/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-ab316310'>ab316310</a>) at 1/1000 dilution

Lane 1:

Untreated Mouse striatum tissue lysate (untreated membrane) at 20 µg

Lane 2:

Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

Untreated Mouse striatum tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Lane 4:

Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 58 kDa,36 kDa

false

Exposure time: 136s

• WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID : 17157277).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (ab137869) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (<a href='/en-us/products/primary-antibodies/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-ab316310'>ab316310</a>) at 1/1000 dilution

Lane 1:

Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (untreated membrane) at 20 µg

Lane 3:

PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 58 kDa,36 kDa

false

Exposure time: 3s

• WB

Unknown

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 1/100000 dilution

All lanes:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 58 kDa

false

• WB

Lab

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 0.03 µg/mL

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false