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Rabbit Recombinant Monoclonal Tyrosine Hydroxylase antibody. Neuron marker. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 69 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869), expandable thumbnail
  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIHC-PWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Expected
Not recommended
Mouse
Not recommended
Tested
Tested
Expected
Not recommended
Rat
Not recommended
Tested
Tested
Tested
Not recommended
Pig
Not recommended
Predicted
Predicted
Predicted
Not recommended

Not recommended
Not recommended

Species

Rat, Mouse, Human, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

For unpurified use at 1/10000 - 1/50000.

Species

Rat

Dilution info

1/5000

Notes

For unpurified use at 1/10000 - 1/50000.

Species

Human

Dilution info

1/5000

Notes

For unpurified use at 1/10000 - 1/50000.

Predicted
Predicted

Species

Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

For unpurified use at 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Plays an important role in the physiology of adrenergic neurons (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).

Alternative names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TH, TYH

Recommended products

Rabbit Recombinant Monoclonal Tyrosine Hydroxylase antibody. Neuron marker. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 69 publications.

Alternative names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TH, TYH

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1532Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The human recommendation is based on the WB result. This antibody may not be suitable for IHC with human samples.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Associated diseases and disorders

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail
    Image courtesy of Carl Hobbs, Kings College London, U.K.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    ab137869 staining Tyrosine Hydroxylase in rat brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21°C followed by incubation with the primary antibody for 2 hours at 1/1000. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1:500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling Tyrosine Hydroxylase with Purified ab137869 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 0.03 µg/mL

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Rat brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling Tyrosine Hydroxylase with Purified ab137869 at 1:500 dilution (1.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail
    Image courtesy of Carl Hobbs, Kings College London, U.K.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    ab137869 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked with 1% B.S.A. for 10 minutes at 21°C followed by incubation with the primary antibody for 2 hours at 1/800. A biotin-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/250 dilution.

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Blocking and diluting buffer : 5% NFDM/TBST

    All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 0.1 µg/mL

    All lanes: Human adrenal gland lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 1/100000 dilution

    All lanes: PC12 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 58 kDa

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail
    This image is courtesy of a customer review submitted by Andre Antunes

    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Blocked with 5% milk for 1 hour at 25°C.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869) at 1/5000 dilution

    Lane 1: SH-SY5Y cell lysate - transduced with AAV vector expressing human TH at 20000 Cells

    Lane 2: SH-SY5Y cell lysate - non-infected at 20000 Cells

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Immunohistochemical analysis of formalin fixed paraffin embedded human cortex labelling Tyrosine Hydroxylase with ab137869 at a concentration of 0.2 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab137869 anti-Tyrosine Hydroxylase [EP1532Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labelling Tyrosine Hydroxylase antibody with ab137869 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with anti-rabbit HQ, anti-HQ HRP followed by ChromoMap DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab137869 anti-Tyrosine Hydroxylase antibody [EP1532Y] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID: 17157277).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (ab137869) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/1000 dilution

    Lane 1: Untreated Mouse striatum tissue lysate (untreated membrane) at 20 µg

    Lane 2: Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 3: Untreated Mouse striatum tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Lane 4: Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58 kDa, 36 kDa

    Exposure time: 136s

  • Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)

    Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID: 17157277).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (ab137869) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/1000 dilution

    Lane 1: Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (untreated membrane) at 20 µg

    Lane 3: PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58 kDa, 36 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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