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Goat Recombinant Monoclonal Tyrosine Hydroxylase antibody. Suitable for IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (AB317785), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (AB317785), expandable thumbnail

Key facts

Isotype
IgG
Host species
Goat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed:30936473).

Additional Targets

TH

Alternative names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

Recommended products

Goat Recombinant Monoclonal Tyrosine Hydroxylase antibody. Suitable for IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1532Y
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker ab137869). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Associated diseases and disorders

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (ab317785), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (ab317785)

    This image was produced using Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free ab317795, the same antibody clone but in a different formulation.

    Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded mouse brain (positive) and mouse liver (negative).

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free ab317795 at 1/500 dilution, and then incubated for 1 hour with Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (ab317785), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (ab317785)

    This image was produced using Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free ab317795, the same antibody clone but in a different formulation.

    Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative).

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free ab317795 at 1/100 dilution, and then incubated for 1 hour with Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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