AB112

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Name: Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) | Abcam
Brand: Abcam Limited
SKU: AB112
Price: 400 USD
Availability: InStock
Rating: 5 (37 reviews)

Lab Essentials
What is this?

(37 Reviews)

(515 Publications)

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) is a rabbit polyclonal antibody detecting Tyrosine Hydroxylase in Western Blot, IHC-P. Suitable for Rat.

- Over 380 publications
- Trusted since 1999

View Alternative Names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

IHC-P

AbReview7140****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.

The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrus

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).

Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.

IHC-P

AbReview6435****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.

The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

This image is courtesy of an anonymous Abreview

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution

All lanes:

rat caudate lysate at 10 µg

Predicted band size: 58 kDa

false

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution

All lanes:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

Predicted band size: 58 kDa

false

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution

All lanes:

rat striatal lysate at 10 µg

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Rat TH.

P04177

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/750", "IHCP-species-notes": "<p></p>" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB.

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) has been cited over 389 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) has high sensitivity and specificity.

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) has 37 independent reviews from customers.

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) specifically detects Tyrosine Hydroxylase (UniProt ID: P04177; Molecular weight: 56kDa) and is sold in 100 µL selling sizes.

ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan but with lower specificity (PubMed : 10933781, PubMed : 11922614). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).

See full target information TH

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Publications (515)

Recent publications for all applications. Explore the full list and refine your search

eNeuro 12: PubMed41057264

2025

The Ventral Pallidum Innervates a Distinct Subset of Midbrain Dopamine Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia J Yang,Hannah B Elam,Kayla Lilly,Alexandra M McCoy,Valeriia Klepikova,Stephanie M Perez,Daniel J Lodge

Translational psychiatry 15:371 PubMed41053038

2025

Optogenetic stimulation of midbrain dopaminergic neurons rescues hippocampal synaptic plasticity deficits in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Ficchì,Emma Cauzzi,Livia La Barbera,Maria Luisa De Paolis,Gilda Loffredo,Elena Spoleti,Irene Ferrari,Luana Saba,Filippo Biamonte,Annalisa Nobili,Paraskevi Krashia,Marcello D'Amelio

iScience 28:113541 PubMed41050933

2025

Reproducibility of PD patient-specific midbrain organoid data for disease modeling.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Zuccoli,Haya Al Sawaf,Mona Tuzza,Sarah L Nickels,Alise Zagare,Jens C Schwamborn

Behavioral and brain functions : BBF 21:29 PubMed41024062

2025

PHLPP1 deficiency alleviates dopaminergic neurodegeneration and represses neuroinflammation in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilin Chen,Yuan Liu,Jinyue Zhao,Xin Zhou,Yudi Han,Zikai Zhou,Huazheng Liang,Yong Bi

NPJ precision oncology 9:311 PubMed40957885

2025

Targeted ferroptosis induction enhances chemotherapy efficacy in chemoresistant neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana Mañas,Alexandra Seger,Aleksandra Adamska,Kyriaki Smyrilli,Joachim T Siaw,Katarzyna Radke,Erick A Muciño-Olmos,Oscar C Bedoya-Reina,Javanshir Esfandyari,Kristina Aaltonen,Daniel Bexell

Frontiers in cell and developmental biology 13:1608400 PubMed40837190

2025

Epigenetic regulation of the respiratory chain by a mitochondrial distress-related redox signal.

Applications

Unspecified application

Species

Unspecified reactive species

Marius W Baeken,Ankush Borlepawar,Philipp Kötzner,Holger Richly,Christian Behl,Bernd Moosmann,Parvana Hajieva

NPJ Parkinson's disease 11:242 PubMed40804075

2025

Early synaptic changes and reduced brain connectivity in PD-like mice with depressive phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Lluis Miquel-Rio,Judith Jericó-Escolar,Unai Sarriés-Serrano,Claudia Yanes-Castilla,María Torres-López,Uxia Argibay,Verónica Paz,Carme Casal,Emma Muñoz-Moreno,Xavier López-Gil,Analia Bortolozzi

PLoS biology 23:e3003289 PubMed40705829

2025

Regulation of working memory switches from striatal dopamine D2-receptor to D1-receptor neurons under high cognitive load.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Jun Chen,Fei Li,Xinyue Zhao,Long Chen,Jin Xue,Zhimo Yao,Zuobin Gan,Xiaoyue Lian,Zhenghao Liu,Luyao Tong,Qingshan Yan,Linan Qiu,Qin Wang,Jiang-Fan Chen,Zhihui Li

Frontiers in molecular neuroscience 18:1574219 PubMed40528874

2025

A Na1.8 mouse enabling selective intersectional targeting of low threshold C fiber mechanoreceptors and nociceptors.

Applications

Unspecified application

Species

Unspecified reactive species

John C Y Chen,Lech Kaczmarczyk,Walker S Jackson,Max Larsson

Cellular and molecular neurobiology 45:54 PubMed40445293

2025

Fibrinogen and Complement Factor H Induce Parkinsonian and Cognitive Impairment-Like Features in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aditi Naskar,Mahesha Sachin,Senjuti Sengupta,Pallavi Bhadrachalam,Shantala Hegde,Ravi Yadav,Pramod Kumar Pal,Phalguni Anand Alladi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB112)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (515)

Product promise