AB137721

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Name: Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab137721) | Abcam
Brand: Abcam Limited
SKU: AB137721
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal Tyrosine Hydroxylase antibody. Suitable for WB, IHC-Fr, ICC/IF, IHC-P and reacts with Mouse, Rat, Human, Zebrafish samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TH aa 150-500.

View Alternative Names

TYH, TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase

10 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunofluorescence analysis of U-87 MG cells fixed in 4% paraformaldehyde at RT for 15 min labelling Tyrosine Hydroxylase protein at cytoplasm using ab137721 at a 1/400 dilution (Green). beta Tubulin 3/ TUJ1 was stained by anti beta Tubulin 3/ TUJ1 antibody at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for Tyrosine Hydroxylase protein with ab137721 at a 1/1000 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for Tyrosine Hydroxylase protein at cytoplasm with ab137721 at a 1/2500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemical analysis of Frozen sectioned adult mouse retina staining Tyrosine Hydroxylase with ab137721 at a dilution of 1/250 (Green). beta Tubulin 3 stained by anti beta Tubulin 3/ TUJ1 antibody diluted at 1/250 dilution (Red). The nuclear staining is DAPI (Blue).

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab137721) at 1/500 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

PC-3 whole cell lysate at 30 µg

Lane 3:

U87-MG whole cell lysate at 30 µg

Lane 4:

SK-N-SH whole cell lysate at 30 µg

Predicted band size: 58 kDa

false

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab137721) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Observed band size: 40 kDa

false

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab137721) at 1/500 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Mouse muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 40 kDa

false

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab137721) at 1/500 dilution

All lanes:

Zebrafish tissue extract at 30 µg

Observed band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

WB, ICC/IF, IHC-P, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human TH aa 150-500. The exact immunogen used to generate this antibody is proprietary information.

P07101

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol." }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed : 15287903, PubMed : 1680128, PubMed : 17391063, PubMed : 24753243, PubMed : 34922205, PubMed : 8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).. Isoform 5. Lacks catalytic activity.. Isoform 6. Lacks catalytic activity.

See full target information TH

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:53 PubMed38383441

2024

Integration of National Health Insurance claims data and animal models reveals fexofenadine as a promising repurposed drug for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Bong Kim,Yujeong Kim,Soo-Jeong Kim,Tae-Young Ha,Dong-Kyu Kim,Dong Won Kim,Minyoung So,Seung Ho Kim,Hyun Goo Woo,Dukyong Yoon,Sang Myun Park

3 Biotech 13:149 PubMed37131964

2023

Swimming training and herbal nanoformulations as natural remedies to improve sensory-motor impairment in rat midbrain tumor models: system biology, behavioral test, and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Fariba Farajizadeh,Farzaneh Taghian,Khosro Jalali Dehkordi,Rezvan Mirsafaei Rizi

Oxidative medicine and cellular longevity 2022:7671324 PubMed35936219

2022

miR-494-3p Promotes Erastin-Induced Ferroptosis by Targeting REST to Activate the Interplay between SP1 and ACSL4 in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Ma,Xiaohuan Li,Yongyan Fan,Dawei Yang,Qi Gu,Dongsheng Li,Siyuan Chen,Shaopu Wu,Jinhua Zheng,Hongqi Yang,Xue Li

Frontiers in molecular neuroscience 14:697440 PubMed34305527

2021

Glucagon-Like Peptide-1 Receptor Agonist Ameliorates 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine (MPTP) Neurotoxicity Through Enhancing Mitophagy Flux and Reducing α-Synuclein and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Tsu-Kung Lin,Kai-Jung Lin,Hung-Yu Lin,Kai-Lieh Lin,Min-Yu Lan,Pei-Wen Wang,Tzu-Jou Wang,Feng-Sheng Wang,Po-Chin Tsai,Chia-Wei Liou,Jiin-Haur Chuang

eLife 10: PubMed34042046

2021

SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fan,Shuang Tang,Xiaojuan Fan,Yi Fang,Xiaojiang Xu,Leping Li,Jian Xu,Jian-Liang Li,Zefeng Wang,Xiaoling Li

Frontiers in neuroanatomy 13:73 PubMed31379520

2019

An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Wonn S Pyon,Daniel T Gray,Carol A Barnes

Journal of vascular research 54:259-271 PubMed28810262

2017

Fetal Sheep Mesenteric Resistance Arteries: Functional and Structural Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Julia J Müller,Matthias Schwab,Charles R Rosenfeld,Iwa Antonow-Schlorke,Peter W Nathanielsz,Florian Rakers,Harald Schubert,Otto W Witte,Sven Rupprecht

The international journal of neuropsychopharmacolo 17:1647-58 PubMed24763081

2014

Long-lasting alterations in 5-HT2A receptor after a binge regimen of methamphetamine in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yi Chiu,Ming-Huan Chan,Mei-Yi Lee,Shao-Tsu Chen,Zih-Yi Zhan,Hwei-Hsien Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB137721)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise