Anti-Tyrosine Hydroxylase antibody is a rabbit polyclonal antibody that is used to detect Tyrosine Hydroxylase in ICC/IF, IHC-FoFr, IHC-Fr, Western blot. Suitable for Mouse, Rat samples.
- Cited in over 75 publications
- Tried and trusted by researchers since 2000
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- Nature communications 14:78012023Dbh catecholaminergic cardiomyocytes contribute to the structure and function of the cardiac conduction system in murine heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyi Sun,Alexander Grassam-Rowe,Zhaoli Pu,Yangpeng Li,Huiying Ren,Yanru An,Xinyu Guo,Wei Hu,Ying Liu,Yuqing Zheng,Zhu Liu,Kun Kou,Xianhong Ou,Tangting Chen,Xuehui Fan,Yangyang Liu,Shu Tu,Yu He,Yue Ren,Ao Chen,Zhouchun Shang,Zhidao Xia,Lucile Miquerol,Nicola Smart,Henggui Zhang,Xiaoqiu Tan,Weinian Shou,Ming LeiPubMed 38016975
- Molecular neurobiology 61:3397-34082023MiR-184 Mediated the Expression of ZNF865 in Exosome to Promote Procession in the PD Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Liu,Yang Wang,Jing-Wen Li,Xiaoyan Zhu,Hai-Song Jiang,Hong Zhao,Li-Ming ZhangPubMed 37989982
- International journal of molecular sciences 24:2023Low-Field Magnetic Stimulation Alleviates MPTP-Induced Alterations in Motor Function and Dopaminergic Neurons in Male Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSathiya Sekar,Yanbo Zhang,Hajar Miranzadeh Mahabadi,Benson Buettner,Changiz TaghibiglouPubMed 37373475
- Journal of neuroinflammation 20:1172023Dysbiosis of gut microbiota inhibits NMNAT2 to promote neurobehavioral deficits and oxidative stress response in the 6-OHDA-lesioned rat model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianjun Yu,Jianhong Meng,Zhengwei Qin,Yuan Yu,Yingxin Liang,Yanjun Wang,Dongmei MinPubMed 37208728
- International journal of molecular sciences 24:2023The Biological Behaviors of Neural Stem Cell Affected by Microenvironment from Host Organotypic Brain Slices under Different Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Jiao,Li Wang,Zhichao Zhang,Xinlin Chen,Haixia Lu,Yong LiuPubMed 36835592
- Translational psychiatry 13:172023Fecal microbiota transplantation improves VPA-induced ASD mice by modulating the serotonergic and glutamatergic synapse signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJifeng Wang,Yuan Cao,Weiliang Hou,Dexi Bi,Fang Yin,Yaohui Gao,Dengfeng Huang,Yingying Li,Zhan Cao,Yinmei Yan,Jianhua Zhao,Dewu Kong,Xiaoqiong Lv,Linsheng Huang,Hui Zhong,Chunyan Wu,Qiyi Chen,Rong Yang,Qing Wei,Huanlong QinPubMed 36670104
- Frontiers in aging neuroscience 14:10203212022Deep brain stimulation in the globus pallidus alleviates motor activity defects and abnormal electrical activities of the parafascicular nucleus in parkinsonian rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinlu Xie,Zheng Chen,Tingting He,Hengya Zhu,Tingyu Chen,Chongbin Liu,Xuyan Fu,Hong Shen,Tao LiPubMed 36248005
- Experimental and therapeutic medicine 24:6352022Effects of Brain Factor‑7® against motor deficit and oxidative stress in a mouse model of MPTP‑induced Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTae-Kyeong Lee,Jae-Chul Lee,Dae Won Kim,Ji-Won Lee,Sung-Su Kim,Hyung-Il Kim,Myoung Cheol Shin,Jun Hwi Cho,Moo-Ho Won,Soo Young ChoiPubMed 36160902
- Cancers 14:2022Differential HIF2α Protein Expression in Human Carotid Body and Adrenal Medulla under Physiologic and Tumorigenic Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucía Celada,Tamara Cubiella,Jaime San-Juan-Guardado,Andrés San José Martínez,Nuria Valdés,Paula Jiménez-Fonseca,Ignacio Díaz,Jose María Enguita,Aurora Astudillo,Enol Álvarez-González,Luisa María Sierra,María-Dolores ChiaraPubMed 35740651
- iScience 25:1043882022Dopamine modulates visual threat processing in the superior colliculus via D2 receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuentin Montardy,Zheng Zhou,Lei Li,Qingning Yang,Zhuogui Lei,Xiaolong Feng,Shanping Chen,Qianqian Shi,Huiqi Zhang,Shuran Chen,Zhijian Zhang,Binghao Zhao,Fuqiang Xu,Zhonghua Lu,Liping WangPubMed 35633939
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 7.4
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat TH aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P04177
Reactivity data
Select an application| WB | IHC-FoFr | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Tested | Tested | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Chimpanzee, Macaque monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed:30936473).
Alternative names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th
Recommended products
Anti-Tyrosine Hydroxylase antibody is a rabbit polyclonal antibody that is used to detect Tyrosine Hydroxylase in ICC/IF, IHC-FoFr, IHC-Fr, Western blot. Suitable for Mouse, Rat samples.
- Cited in over 75 publications
- Tried and trusted by researchers since 2000
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 7.4
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat TH aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P04177
- Purity
- Whole antiserum
- Specificity
Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-Fr, WB in mouse samples.
Anti-Tyrosine Hydroxylase antibody (ab6211) has been cited over 77 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody (ab6211) has high sensitivity and specificity.
Anti-Tyrosine Hydroxylase antibody (ab6211) has 4 independent reviews from customers.
Anti-Tyrosine Hydroxylase antibody (ab6211) specifically detects Tyrosine Hydroxylase (UniProt ID: P04177; Molecular weight: 56kDa) and is sold in a 50 µL selling size.
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
- Biological function summary
Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.
- Pathways
Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.
- Associated diseases and disorders
Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)
ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of Orexin A (bovine, human, mouse, rat), Neuropeptide involved in feeding and sleep ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (Normal Goat Serum ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab6211)
Tyrosine Hydroxylase Western blot staining of PC12 cell lysates using rabbit Anti-Tyrosine Hydroxylase antibody
Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab6211) at 1/100 dilution
All lanes: PC12 cell lysates at 6 µg
Performed under reducing conditions.
Predicted band size: 58 kDa
Image courtesy of QBM Cell Sciences, Ottawa, CanadaImmunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
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