Anti-Tyrosine Hydroxylase antibody is a chicken polyclonal antibody that is used to detect Tyrosine Hydroxylase in IHC-FrFl, IHC-P. Suitable for Mouse samples.
- Tried and trusted by researchers since 2009
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- The Journal of neuroscience : the official journal of the Society for Neuroscience 44:2024Frequency Specific Optogenetic Stimulation of the Locus Coeruleus Induces Task-Relevant Plasticity in the Motor Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Tzu Tseng,Hailey F Welch,Ashley L Gi,Erica Mina Kang,Tanushree Mamidi,Sahiti Pydimarri,Kritika Ramesh,Alfredo Sandoval,Jonathan E Ploski,Catherine A ThornPubMed 38124020
- Acta neuropathologica communications 11:2012023Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVijay Singh,Marissa A Menard,Geidy E Serrano,Thomas G Beach,Hien T Zhao,Alexis Riley-DiPaolo,Nitya Subrahmanian,Matthew J LaVoie,Laura A Volpicelli-DaleyPubMed 38110990
- Communications biology 6:12112023Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc FerrerPubMed 38017066
- Nature communications 14:75292023Brain-to-gut trafficking of alpha-synuclein by CD11c cells in a mouse model of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRhonda L McFleder,Anastasiia Makhotkina,Janos Groh,Ursula Keber,Fabian Imdahl,Josefina Peña Mosca,Alina Peteranderl,Jingjing Wu,Sawako Tabuchi,Jan Hoffmann,Ann-Kathrin Karl,Axel Pagenstecher,Jörg Vogel,Andreas Beilhack,James B Koprich,Jonathan M Brotchie,Antoine-Emmanuel Saliba,Jens Volkmann,Chi Wang IpPubMed 37981650
- Nature communications 14:72952023Knockout or inhibition of USP30 protects dopaminergic neurons in a Parkinson's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTracy-Shi Zhang Fang,Yu Sun,Andrew C Pearce,Simona Eleuteri,Mark Kemp,Christopher A Luckhurst,Rachel Williams,Ross Mills,Sarah Almond,Laura Burzynski,Nóra M Márkus,Christopher J Lelliott,Natasha A Karp,David J Adams,Stephen P Jackson,Jin-Feng Zhao,Ian G Ganley,Paul W Thompson,Gabriel Balmus,David K SimonPubMed 37957154
- Experimental & molecular medicine 55:2357-23752023PLCγ1 in dopamine neurons critically regulates striatal dopamine release via VMAT2 and synapsin III.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye Yun Kim,Jieun Lee,Hyun-Jin Kim,Byeong Eun Lee,Jaewook Jeong,Eun Jeong Cho,Hyun-Jun Jang,Kyeong Jin Shin,Min Ji Kim,Young Chan Chae,Seung Eun Lee,Kyungjae Myung,Ja-Hyun Baik,Pann-Ghill Suh,Jae-Ick KimPubMed 37907739
- Laboratory animal research 39:202023The mesocortical dopaminergic system cannot explain hyperactivity in an animal model of attention deficit hyperactivity disorder (ADHD)- Spontaneously hypertensive rats (SHR).Applications:Unspecified applicationReactive species:Unspecified reactive speciesAysegul Gungor Aydin,Esat AdiguzelPubMed 37710339
- Cells 12:2023The Pesticide Chlordecone Promotes Parkinsonism-like Neurodegeneration with Tau Lesions in Midbrain Cultures and Worms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValeria Parrales-Macias,Patrick P Michel,Aurore Tourville,Rita Raisman-Vozari,Stéphane Haïk,Stéphane Hunot,Nicolas Bizat,Annie LannuzelPubMed 37174736
- Molecular therapy. Methods & clinical development 29:381-3942023Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants .Applications:Unspecified applicationReactive species:Unspecified reactive speciesJana Rájová,Marcus Davidsson,Martino Avallone,Morgan Hartnor,Patrick Aldrin-Kirk,Tiago Cardoso,Sara Nolbrant,Annelie Mollbrink,Petter Storm,Andreas Heuer,Malin Parmar,Tomas BjörklundPubMed 37251982
- International journal of molecular sciences 24:2023DDC-Promoter-Driven Chemogenetic Activation of SNpc Dopaminergic Neurons Alleviates Parkinsonian Motor Symptoms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Chan Seo,Yeon Ha Ju,Jin-Ju Seo,Soo-Jin Oh,C Justin Lee,Seung Eun Lee,Min-Ho NamPubMed 36768816
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TH conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P07101
Reactivity data
Select an application| WB | IHC-P | IHC-FrFl | |
|---|---|---|---|
| Mouse | Not recommended | Tested | Tested |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes because of high background. However a band at 58kDa is present and this band can be inhibited by the immunogen peptide |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
IHC-FrFl
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:15287903, PubMed:1680128, PubMed:17391063, PubMed:24753243, PubMed:34922205, PubMed:8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). Isoform 5. Lacks catalytic activity. Isoform 6. Lacks catalytic activity.
Alternative names
TYH, TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase
Recommended products
Anti-Tyrosine Hydroxylase antibody is a chicken polyclonal antibody that is used to detect Tyrosine Hydroxylase in IHC-FrFl, IHC-P. Suitable for Mouse samples.
- Tried and trusted by researchers since 2009
Key facts
- Isotype
- IgY
- Host species
- Chicken
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TH conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P07101
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Two different affinity-purified anti-peptide antibodies were combined to make this product. After repeated injections of synthetic peptides into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized.The concentrations of both antibodies were 100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200 ug/ml.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Tyrosine Hydroxylase antibody (ab76442) is a chicken polyclonal antibody and is validated for use in IHC-FrFl, IHC-P in rat samples.
Anti-Tyrosine Hydroxylase antibody (ab76442) has been cited over 160 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody (ab76442) has high sensitivity and specificity.
Anti-Tyrosine Hydroxylase antibody (ab76442) has 12 independent reviews from customers.
Anti-Tyrosine Hydroxylase antibody (ab76442) specifically detects Tyrosine Hydroxylase (UniProt ID: P07101; Molecular weight: 59kDa) and is sold in 250 µL selling sizes.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
- Biological function summary
Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.
- Pathways
Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.
- Associated diseases and disorders
Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)
Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
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