Rabbit Recombinant Multiclonal Tyrosine Hydroxylase antibody. Carrier free. Suitable for WB, IHC-P, IP, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunoprecipitation-img424090.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253)")
![Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunoprecipitation-img424089.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253)")
![Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunoprecipitation-img424088.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424058.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424063.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (AB315253)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | IHC-Fr | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Expected | Expected |
| Rat | Tested | Tested | Tested | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:15287903, PubMed:1680128, PubMed:17391063, PubMed:24753243, PubMed:34922205, PubMed:8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). Isoform 5. Lacks catalytic activity. Isoform 6. Lacks catalytic activity.
Alternative names
TYH, TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase
Recommended products
Rabbit Recombinant Multiclonal Tyrosine Hydroxylase antibody. Carrier free. Suitable for WB, IHC-P, IP, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1085
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for human ICC and FC-intra. Unsuitable for mouse IHC frozen.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315253 is the carrier-free version of Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252.
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
- Biological function summary
Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.
- Pathways
Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.
- Associated diseases and disorders
Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
19 product images
![Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunoprecipitation-img424090.jpg "Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase was immunoprecipitated from 0.35 mg Rat adrenal grand tissue lysate with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat adrenal grand tissue lysate
Lane 2: Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 IP in Rat adrenal grand tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 in rat adrenal grand tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] (Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252) at 1/30 dilution
All lanes: Rat adrenal grand tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunoprecipitation-img424089.jpg "Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase was immunoprecipitated from 0.35 mg Mouse adrenal grand tissue lysate with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse adrenal grand tissue lysate
Lane 2: Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 IP in Mouse adrenal grand tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 in mouse adrenal grand tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] (Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252) at 1/30 dilution
All lanes: Mouse adrenal grand tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunoprecipitation-img424088.jpg "Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase was immunoprecipitated from 0.35 mg Human adrenal grand tissue lysate with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human adrenal grand tissue lysate
Lane 2: Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 IP in Human adrenal grand tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 in human adrenal grand tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase antibody [RM1085] (Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252) at 1/30 dilution
All lanes: Human adrenal grand tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424058.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human adrenal gland. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424063.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human lung. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--western-blot-img424047.jpg "Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] (Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252) at 1/5000 dilution
All lanes: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 58 kDa
Exposure time: 4s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424061.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat striatum. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-frozen-sections-img424098.jpg "Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh) tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/50 (9.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Negative control: Confocal image showing no staining on rat lung. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424062.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424060.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-frozen-sections-img424097.jpg "Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat striatum (fresh) tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/50 (9.36 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on rat striatum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--flow-cytometry-intracellular-img424093.jpg "Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 dilution (0.01 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--western-blot-img424046.jpg "Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver and lung.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] (Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252) at 1/1000 dilution
Lane 1: Human adrenal grand tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Human lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 58 kDa, 36 kDa
Exposure time: 48s
![Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunocytochemistry-immunofluorescence-img424085.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/100 (4.68 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic staining in PC-12 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424065.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat lung. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424064.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse lung. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424057.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human striatum tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human striatum. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424059.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse adrenal gland tissue labeling Tyrosine Hydroxylase with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 at 1/5000 (0.094 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse adrenal gland. The section was incubated with Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--western-blot-img424045.jpg "Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)
This data was developed using Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver and lung.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1 and 3-9: 180 seconds; Lane 2: 59 seconds
All lanes: Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] (Anti-Tyrosine Hydroxylase antibody [RM1085] ab315252) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse adrenal grand tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse lung tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat adrenal grand tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
Lane 8: Rat lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 58 kDa, 36 kDa
![Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--western-blot-img424047.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424061.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-frozen-sections-img424098.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424062.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424060.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-frozen-sections-img424097.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--flow-cytometry-intracellular-img424093.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--western-blot-img424046.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunocytochemistry-immunofluorescence-img424085.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424065.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424064.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424057.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img424059.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
![Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-antibody-rm1085-bsa-and-azide-free-ab315253--western-blot-img424045.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tyrosine Hydroxylase antibody [RM1085] - BSA and Azide free (ab315253)")
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