AB254031

Anti-Tyrosine Hydroxylase (phospho S31) antibody

Name: Anti-Tyrosine Hydroxylase (phospho S31) antibody (ab254031) | Abcam
Brand: Abcam Limited
SKU: AB254031
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Tyrosine Hydroxylase phospho S31 antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Rat Th phospho S31.

View Alternative Names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH

1 Images

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase (phospho S31) antibody (AB254031)

All lanes:

Western blot - Anti-Tyrosine Hydroxylase (phospho S31) antibody (ab254031) at 1/1000 dilution

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate in the abscence of okadaic acid

Lane 2:

PC-12 cell lysate in the prescence of okadaic acid (1 µM for 60 mins)

Predicted band size: 58 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Rat Th phospho S31. The exact immunogen used to generate this antibody is proprietary information.

P04177

Specificity

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser³¹.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Storage buffer

pH: 7.5 Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan but with lower specificity (PubMed : 10933781, PubMed : 11922614). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).

See full target information Th phospho S31

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:59 PubMed37013604

2023

Upregulation of carbonic anhydrase 1 beneficial for depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Cheng,Yue Wang,Yong He,Yu Tian,Junjie Li,Chong Chen,Xingzhe Xu,Zhonghao Wu,Heming Yu,Xiangyu Chen,Yili Wu,Weihong Song,Zhifang Dong,Huatai Xu,Peng Xie

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Tyrosine Hydroxylase (phospho S31) antibody

Western blot - Anti-Tyrosine Hydroxylase (phospho S31) antibody (AB254031)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise