Skip to main content

Rabbit Recombinant Monoclonal Tyrosine Hydroxylase phospho S40 antibody. Suitable for Dot, WB, IHC-P, IP and reacts with Synthetic peptide - Mouse, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (AB316310), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (AB316310), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (AB316310), expandable thumbnail
  • Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (AB316310), expandable thumbnail
  • Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (AB316310), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIHC-PIPICC/IFFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Tested
Tested
Not recommended
Not recommended
Synthetic peptide - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Synthetic peptide - Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Synthetic peptide - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Synthetic peptide - Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Synthetic peptide - Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed:30936473).

Alternative names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

Recommended products

Rabbit Recombinant Monoclonal Tyrosine Hydroxylase phospho S40 antibody. Suitable for Dot, WB, IHC-P, IP and reacts with Synthetic peptide - Mouse, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26191-5
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.

Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Associated diseases and disorders

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Immunohistochemical analysis of paraffin-embedded Rat adrenal gland tissue labeling Tyrosine Hydroxylase (phospho S40) with ab316310 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat adrenal gland without alkaline phosphatase treatment (image A), no signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab316310 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Tyrosine Hydroxylase (phospho S40) with ab316310 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse striatum without alkaline phosphatase treatment (image A), no signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab316310 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Immunohistochemical analysis of paraffin-embedded Mouse adrenal gland tissue labeling Tyrosine Hydroxylase (phospho S40) with ab316310 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse adrenal gland without alkaline phosphatase treatment (image A), no signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab316310 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Tyrosine Hydroxylase (phospho S40) was immunoprecipitated from 0.35 mg Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate with ab316310 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316310 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate

    Lane 2: ab316310 IP in Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316310 in Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate

    All lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310) at 1/30 dilution

    All lanes: Mouse striatum treated with 10?M forskolin for 10 minutes whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 48s

  • Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Tyrosine Hydroxylase (phospho S40) was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30μM forskolin for 60 minutes whole cell lysate with ab316310 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316310 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30μM forskolin for 60 minutes whole cell lysate

    Lane 2: ab316310 IP in PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30μM forskolin for 60 minutes whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316310 in PC-12 treated with 30μM forskolin for 60 minutes whole cell lysate

    All lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310) at 1/30 dilution

    All lanes: PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30?M forskolin for 60 minutes whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID: 17157277).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker ab137869) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310) at 1/1000 dilution

    Lane 1: Untreated Mouse striatum tissue lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 2: Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 3: Untreated Mouse striatum tissue lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58 kDa, 36 kDa

    Exposure time: 136s

  • Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID: 17157277).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker ab137869) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310) at 1/1000 dilution

    Lane 1: Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 2: PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 3: PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58 kDa, 36 kDa

    Exposure time: 3s

  • Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310), expandable thumbnail

    Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310)

    Dot blot analysis of Tyrosine Hydroxylase (phospho S40) using ab316310 at 1:1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    All lanes: Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (ab316310) at 1/1000 dilution

    Lane 1: Tyrosine Hydroxylase (phospho S40) peptide a with 5% NFDM/TBST

    Lane 2: Tyrosine Hydroxylase (phospho S40) peptide b with 5% NFDM/TBST

    Lane 3: Tyrosine Hydroxylase non-phospho peptide c with 5% NFDM/TBST

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com