Rabbit Recombinant Monoclonal Tyrosine Hydroxylase phospho S40 antibody. Carrier free. Suitable for Dot, WB, IHC-P, IP and reacts with Synthetic peptide - Mouse, Mouse, Rat samples.
Images
![Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--dot-blot-img427833.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427842.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427841.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427840.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311)")
![Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunoprecipitation-img427872.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (AB316311)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | IP | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Expected | Tested | Tested | Tested | Not recommended | Not recommended |
| Synthetic peptide - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide - Mouse, Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed:30936473).
Alternative names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th
Recommended products
Rabbit Recombinant Monoclonal Tyrosine Hydroxylase phospho S40 antibody. Carrier free. Suitable for Dot, WB, IHC-P, IP and reacts with Synthetic peptide - Mouse, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26191-5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316311 is the carrirer-free version of Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
- Biological function summary
Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.
- Pathways
Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.
- Associated diseases and disorders
Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--dot-blot-img427833.jpg "Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
Tyrosine Hydroxylase (phospho S40) Dot Blot staining using rabbit Anti-Tyrosine Hydroxylase (phospho S40) antibody
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Dot blot analysis of Tyrosine Hydroxylase (phospho S40) using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1:1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
All lanes: Dot Blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/1000 dilution
Lane 1: Tyrosine Hydroxylase (phospho S40) peptide a
Lane 2: Tyrosine Hydroxylase (phospho S40) peptide b
Lane 3: Tyrosine Hydroxylase non-phospho peptide c
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427842.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat adrenal gland tissue labeling Tyrosine Hydroxylase (phospho S40) with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat adrenal gland without alkaline phosphatase treatment (image A), no signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427841.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Tyrosine Hydroxylase (phospho S40) with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse striatum without alkaline phosphatase treatment (image A), no signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427840.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse adrenal gland tissue labeling Tyrosine Hydroxylase (phospho S40) with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse adrenal gland without alkaline phosphatase treatment (image A), no signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunoprecipitation-img427872.jpg "Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase (phospho S40) was immunoprecipitated from 0.35 mg Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate
Lane 2: Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 IP in Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 in Mouse striatum treated with 10μM forskolin for 10 minutes whole cell lysateAll lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/30 dilution
All lanes: Mouse striatum treated with 10?M forskolin for 10 minutes whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 48s
![Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunoprecipitation-img427829.jpg "Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase (phospho S40) was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30μM forskolin for 60 minutes whole cell lysate with Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30μM forskolin for 60 minutes whole cell lysate
Lane 2: Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 IP in PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30μM forskolin for 60 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310 in PC-12 treated with 30μM forskolin for 60 minutes whole cell lysateAll lanes: Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/30 dilution
All lanes: PC-12 (rat adrenal gland pheochromocytoma cell) treated with 30?M forskolin for 60 minutes whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
![Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--western-blot-img427820.jpg "Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID: 17157277).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker ab137869) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/1000 dilution
Lane 1: Untreated Mouse striatum tissue lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 3: Untreated Mouse striatum tissue lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 4: Mouse striatum treated with 10uM forskolin for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 58 kDa, 36 kDa
Exposure time: 136s
![Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--western-blot-img427819.jpg "Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)
This data was developed using Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310, the same antibody clone in a different buffer formulation.
Tyrosine Hydroxylase (phospho S40) expression can be induced by forskolin treatment (PMID: 17157277).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Tyrosine Hydroxylase antibody - Total protein control (Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker ab137869) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] (Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] ab316310) at 1/1000 dilution
Lane 1: Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 3: PC-12 treated with 30uM forskolin for 60 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 58 kDa, 36 kDa
Exposure time: 3s
![Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--immunoprecipitation-img427829.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
![Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--western-blot-img427820.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
![Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311), expandable thumbnail](https://content.abcam.com/products/images/tyrosine-hydroxylase-phospho-s40-antibody-epr26191-5-bsa-and-azide-free-ab316311--western-blot-img427819.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tyrosine Hydroxylase (phospho S40) antibody [EPR26191-5] - BSA and Azide free (ab316311)")
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