AB150429

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)]

RabMAb
Recombinant
What is this?

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal Tyrosyl tRNA synthetase/TyrRS antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

YARS, YARS1, Tyrosyl-tRNA synthetase, TyrRS

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tyrosyl tRNA synthetase/TyrRS with ab150429 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab150429 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Tyrosyl tRNA synthetase/TyrRS with ab150429 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab150429 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150429 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab150429 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

WB

Unknown

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (AB150429)

All lanes:

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] (ab150429) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

ECV-304 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 59 kDa

false

Carrier free

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [EPR9926(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9926(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosyl tRNA synthetase also known as TyrRS is an enzyme responsible for charging tRNA molecules with the amino acid tyrosine. This process known as aminoacylation is essential for accurate translation of the genetic code during protein synthesis. TyrRS has a molecular weight of approximately 58 kDa. It is expressed in various tissues playing a significant role in both cytoplasmic and mitochondrial protein synthesis in eukaryotes.

Biological function summary

TyrRS plays an important role in ensuring the fidelity of protein translation within cells. It facilitates the correct pairing of tRNA^Tyr with its corresponding tyrosine amino acid contributing to the accuracy of protein production. This enzyme is part of the aminoacyl-tRNA synthetase complex a multi-enzyme complex that catalyzes the attachment of specific amino acids to their appropriate tRNA molecules an important step in the translation process.

Pathways

TyrRS is involved in the protein synthesis pathway where it functions alongside other aminoacyl-tRNA synthetases to help maintain the genetic code's accuracy. Additionally TyrRS is implicated in the aminoacyl-tRNA biosynthesis pathway connecting its activity with enzymes responsible for similar processes. It works closely with related enzymes such as phenylalanyl-tRNA synthetase (PheRS) and tryptophanyl-tRNA synthetase (TrpRS) through these pathways ensuring coordinated protein synthesis.

TyrRS has been connected to certain neurodegenerative diseases and cancer. Mutations in the TYRRS gene have been associated with Charcot-Marie-Tooth disease a hereditary neuropathy. The enzyme's altered function in this context may lead to compromised protein synthesis. Additionally aberrant TyrRS expression has been observed in some types of cancer where it interacts with proteins like angiogenin to potentially promote tumor growth. These connections highlight the enzyme's potential role in disease progression and pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction : tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable) (PubMed : 25533949). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity (PubMed : 25533949). Activity is switched upon resveratrol-binding : resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1 (PubMed : 25533949).

See full target information YARS1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical research 37:418-430 PubMed37977559

2023

Galectin-14 promotes hepatocellular carcinoma tumor growth enhancing heparan sulfate proteoglycan modification.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Gou,Gang Yang,Sujuan Ma,Tong Ding,Luan Sun,Fang Liu,Jin Huang,Wei Gao

The EMBO journal 40:e106696 PubMed33346941

2020

A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Doowon Huh,Maria C Passarelli,Jenny Gao,Shahnoza N Dusmatova,Clara Goin,Lisa Fish,Alexandra M Pinzaru,Henrik Molina,Zhiji Ren,Elizabeth A McMillan,Hosseinali Asgharian,Hani Goodarzi,Sohail F Tavazoie

Frontiers in cellular neuroscience 14:232 PubMed32848623

2020

Altered Sensory Neuron Development in CMT2D Mice Is Site-Specific and Linked to Increased GlyRS Levels.

Applications

Unspecified application

Species

Unspecified reactive species

James N Sleigh,Aleksandra M Mech,Tahmina Aktar,Yuxin Zhang,Giampietro Schiavo

Journal of cancer research and clinical oncology 146:329-342 PubMed31912229

2020

YARS as an oncogenic protein that promotes gastric cancer progression through activating PI3K-Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhang,Xiaoting Lin,Qian Zhao,Yakun Wang,Fangli Jiang,Congcong Ji,Yanyan Li,Jing Gao,Jian Li,Lin Shen

RSC advances 9:9323-9330 PubMed35517706

2019

Discovery of human TyrRS inhibitors by structure-based virtual screening, structural optimization, and bioassays.

Applications

Unspecified application

Species

Unspecified reactive species

Shenzhen Huang,Xiang Wang,Guifeng Lin,Jie Cheng,Xiuli Chen,Weining Sun,Rong Xiang,Yamei Yu,Linli Li,Shengyong Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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