AB50961

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08]

Name: Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) | Abcam
Brand: Abcam Limited
SKU: AB50961
Price: 520 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal Tyrosyl tRNA synthetase/TyrRS antibody. Suitable for ICC, IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human YARS1.

View Alternative Names

YARS, YARS1, Tyrosyl-tRNA synthetase, TyrRS

4 Images

Unknown

Immunocytochemistry - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

Unknown

Immunoprecipitation - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.

All lanes:

Immunoprecipitation - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961)

Predicted band size: 59 kDa

false

Unknown

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

All lanes:

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 59 kDa

Observed band size: 58 kDa

true

Unknown

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

All lanes:

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 50 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 59 kDa

Observed band size: 58 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

YAR5H08

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

ICC, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human YARS1. The exact immunogen used to generate this antibody is proprietary information.

P54577

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/10", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "20 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1136% Disodium hydrogenorthophosphate, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosyl tRNA synthetase also known as TyrRS is an enzyme responsible for charging tRNA molecules with the amino acid tyrosine. This process known as aminoacylation is essential for accurate translation of the genetic code during protein synthesis. TyrRS has a molecular weight of approximately 58 kDa. It is expressed in various tissues playing a significant role in both cytoplasmic and mitochondrial protein synthesis in eukaryotes.

Biological function summary

TyrRS plays an important role in ensuring the fidelity of protein translation within cells. It facilitates the correct pairing of tRNA^Tyr with its corresponding tyrosine amino acid contributing to the accuracy of protein production. This enzyme is part of the aminoacyl-tRNA synthetase complex a multi-enzyme complex that catalyzes the attachment of specific amino acids to their appropriate tRNA molecules an important step in the translation process.

Pathways

TyrRS is involved in the protein synthesis pathway where it functions alongside other aminoacyl-tRNA synthetases to help maintain the genetic code's accuracy. Additionally TyrRS is implicated in the aminoacyl-tRNA biosynthesis pathway connecting its activity with enzymes responsible for similar processes. It works closely with related enzymes such as phenylalanyl-tRNA synthetase (PheRS) and tryptophanyl-tRNA synthetase (TrpRS) through these pathways ensuring coordinated protein synthesis.

TyrRS has been connected to certain neurodegenerative diseases and cancer. Mutations in the TYRRS gene have been associated with Charcot-Marie-Tooth disease a hereditary neuropathy. The enzyme's altered function in this context may lead to compromised protein synthesis. Additionally aberrant TyrRS expression has been observed in some types of cancer where it interacts with proteins like angiogenin to potentially promote tumor growth. These connections highlight the enzyme's potential role in disease progression and pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction : tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) (Probable) (PubMed : 25533949). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity (PubMed : 25533949). Activity is switched upon resveratrol-binding : resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1 (PubMed : 25533949).

See full target information YARS1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

IUBMB life 77:e70030 PubMed40476370

2025

Tyrosine and Phenylalanine Activate Neuronal DNA Repair but Exhibit Opposing Effects on Global Transcription and Adult Female Mice Are Resilient to TyrRS/YARS1 Depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Megha Jhanji,Ashita Bhan,Colin Arrowood,Dina W Yakout,Ankit Shroff,Danielle McManus,Henrietta Gifford,Janay Vacharasin,Sofia B Lizarraga,Taras Y Nazarko,Angela M Mabb,Mathew Sajish

Nature communications 13:3244 PubMed35688816

2022

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Megha Jhanji,Chintada Nageswara Rao,Jacob C Massey,Marion C Hope,Xueyan Zhou,C Dirk Keene,Tao Ma,Michael D Wyatt,Jason A Stewart,Mathew Sajish

PloS one 10:e0142253 PubMed26544075

2015

Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography.

Applications

Species

Human

Seong-Jun Park,Hee-Sung Ahn,Jun Seok Kim,Cheolju Lee

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08]

Immunocytochemistry - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

Immunoprecipitation - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

Western blot - Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (AB50961)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise