Rabbit Polyclonal TZFP/ZBTB32 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB32 aa 1-300.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZBTB32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2Y4
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Chimpanzee | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Target data
Function
DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).
Alternative names
Zinc finger and BTB domain-containing protein 32, FANCC-interacting protein, Fanconi anemia zinc finger protein, Testis zinc finger protein, Zinc finger protein 538, ZNF538, TZFP, FAZF, ZBTB32
Recommended products
Rabbit Polyclonal TZFP/ZBTB32 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB32 aa 1-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZBTB32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2Y4
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TZFP also known as ZBTB32 is a protein encoded by the ZBTB32 gene. This protein weighs approximately 58 kDa and is a member of the BTB/POZ and zinc finger family of transcription factors. It acts as a transcriptional repressor by binding to specific DNA sequences and modulating gene expression. TZFP is expressed in various tissues with notable expression in lymphoid tissues and the thymus where it plays an important role in regulating immune cell function and development.
- Biological function summary
TZFP/ZBTB32 influences cell proliferation and differentiation within the immune system. It is not known to be a part of a major protein complex but interacts directly with chromatin to regulate transcription. Through its action it affects immune responses by controlling the activation and repression of genes important for lymphocyte development and function. TZFP also plays roles in the modulation of immune tolerance and autoimmunity.
- Pathways
TZFP/ZBTB32's function is evident in immune regulatory pathways. It interacts with signaling cascades like the NF-κB pathway where it serves as a modulator of transcriptional responses to inflammatory signals. Additionally TZFP engages with other transcription factors like BCL6 which are critical in B and T cell activities. This regulatory control helps ensure a balanced immune response necessary for maintaining homeostasis.
- Associated diseases and disorders
TZFP/ZBTB32 has been implicated in conditions involving immune dysregulation. For example aberrant expression of TZFP is associated with autoimmune diseases such as systemic lupus erythematosus (SLE). It may also relate to certain lymphomas where its altered expression can contribute to malignant transformation and progression. Through its involvement in these disorders proteins like NF-κB and BCL6 often interact influencing disease pathogenesis and offering potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TZFP/ZBTB32 antibody (ab235306)
All lanes: Western blot - Anti-TZFP/ZBTB32 antibody (ab235306) at 1/5000 dilution
Lane 1: Mouse brain tissue lysate
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TZFP/ZBTB32 antibody (ab235306)
Paraffin-embedded human adrenal gland tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TZFP/ZBTB32 antibody (ab235306)
Paraffin-embedded human testis tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.
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