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AB235306

Anti-TZFP/ZBTB32 antibody

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(1 Publication)

Rabbit Polyclonal TZFP/ZBTB32 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB32 aa 1-300.

View Alternative Names

FAZF, TZFP, ZNF538, ZBTB32, Zinc finger and BTB domain-containing protein 32, FANCC-interacting protein, Fanconi anemia zinc finger protein, Testis zinc finger protein, Zinc finger protein 538

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TZFP/ZBTB32 antibody (AB235306)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TZFP/ZBTB32 antibody (AB235306)

Paraffin-embedded human adrenal gland tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TZFP/ZBTB32 antibody (AB235306)
  • WB

Supplier Data

Western blot - Anti-TZFP/ZBTB32 antibody (AB235306)

All lanes:

Western blot - Anti-TZFP/ZBTB32 antibody (ab235306) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 53 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TZFP/ZBTB32 antibody (AB235306)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TZFP/ZBTB32 antibody (AB235306)

Paraffin-embedded human testis tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZBTB32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2Y4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TZFP also known as ZBTB32 is a protein encoded by the ZBTB32 gene. This protein weighs approximately 58 kDa and is a member of the BTB/POZ and zinc finger family of transcription factors. It acts as a transcriptional repressor by binding to specific DNA sequences and modulating gene expression. TZFP is expressed in various tissues with notable expression in lymphoid tissues and the thymus where it plays an important role in regulating immune cell function and development.
Biological function summary

TZFP/ZBTB32 influences cell proliferation and differentiation within the immune system. It is not known to be a part of a major protein complex but interacts directly with chromatin to regulate transcription. Through its action it affects immune responses by controlling the activation and repression of genes important for lymphocyte development and function. TZFP also plays roles in the modulation of immune tolerance and autoimmunity.

Pathways

TZFP/ZBTB32's function is evident in immune regulatory pathways. It interacts with signaling cascades like the NF-κB pathway where it serves as a modulator of transcriptional responses to inflammatory signals. Additionally TZFP engages with other transcription factors like BCL6 which are critical in B and T cell activities. This regulatory control helps ensure a balanced immune response necessary for maintaining homeostasis.

TZFP/ZBTB32 has been implicated in conditions involving immune dysregulation. For example aberrant expression of TZFP is associated with autoimmune diseases such as systemic lupus erythematosus (SLE). It may also relate to certain lymphomas where its altered expression can contribute to malignant transformation and progression. Through its involvement in these disorders proteins like NF-κB and BCL6 often interact influencing disease pathogenesis and offering potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).
See full target information ZBTB32
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1430891 PubMed39114365

2024

CBX2 enhances the progression and TMZ chemoresistance of glioma via EZH2-mediated epigenetic silencing of PTEN expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wang,Bo Yang,Yingzhao Wang,Shuhan Liu,Changkai Ma,Jianmin Piao,Shiqiang Ma,Dehai Yu,Wei Wu
View all publications
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