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AB122901

Anti-U1-C antibody [4H12]

4

(1 Review)

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(2 Publications)

Rat Monoclonal U1-C antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Snrpc.

View Alternative Names

Snrp1c, U1 small nuclear ribonucleoprotein C, U1 snRNP C, U1-C, U1C

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-U1-C antibody [4H12] (AB122901)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-U1-C antibody [4H12] (AB122901)

ab122901 staining U1-C in Mouse L929 cells by Immunoflourescence (green). Right image shows the cells DNA stained with Hoechst.

Western blot - Anti-U1-C antibody [4H12] (AB122901)
  • WB

Unknown

Western blot - Anti-U1-C antibody [4H12] (AB122901)

All lanes:

Western blot - Anti-U1-C antibody [4H12] (ab122901)

All lanes:

HeLa total cell extract.

Predicted band size: 17 kDa

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Immunoprecipitation - Anti-U1-C antibody [4H12] (AB122901)
  • IP

Unknown

Immunoprecipitation - Anti-U1-C antibody [4H12] (AB122901)

Immunoprecipitation of U1-C with ab122901.
Lane 1 : no extract.
Lane 2 : HeLa total cell extract.
HL and LC : heavy and light chains.

All lanes:

Immunoprecipitation - Anti-U1-C antibody [4H12] (ab122901)

Predicted band size: 17 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

4H12

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Snrpc.

Q62241

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification notes
ab122901 was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by a propriety chromatography processes and filter sterilized.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

U1-C also known as U1 small nuclear ribonucleoprotein C is a component of the U1 snRNP complex. It has a molecular mass of approximately 17 kDa and is expressed in the nucleus of eukaryotic cells. This protein plays an essential role in the spliceosome machinery which is responsible for pre-mRNA splicing. U1-C is part of a highly conserved group of proteins that engage in RNA processing and modification.
Biological function summary

U1-C interacts with U1 snRNA and other proteins to form the U1 snRNP an essential component of the spliceosome complex. The spliceosome removes introns from pre-mRNA facilitating the production of mature mRNA. U1-C stabilizes the interaction between the 5' splice site of pre-mRNA and the snRNP playing a significant part in the accuracy of splicing. Its precise function in RNA stability and processing makes it an important aspect of gene expression regulation.

Pathways

U1-C participates in the splicing pathway ensuring the correct removal of introns during RNA maturation. This protein associates closely with U1 snRNA and other spliceosomal proteins such as U1-70K. It plays a role in the regulation of alternative splicing pathways which can affect gene expression patterns. The U1-C protein indirectly influences processes like cell cycle progression and differentiation through its involvement in RNA splicing.

Malfunction or mutation of U1-C can lead to splicing abnormalities which are linked to diseases such as systemic lupus erythematosus and certain cancers. In lupus autoantibodies target the U1 snRNP complex including U1-C leading to immune system dysfunction. This association highlights the importance of U1-C in maintaining cellular and immune system homeostasis. Additionally aberrant splicing facilitated by altered U1-C function may impact oncogene or tumor suppressor pathways contributing to cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.
See full target information Snrpc
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed37956386

2023

Stress-induced nuclear speckle reorganization is linked to activation of immediate early gene splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Hsu-Min Sung,Johanna Schott,Philipp Boss,Janina A Lehmann,Marius Roland Hardt,Doris Lindner,Joris Messens,Ivan Bogeski,Uwe Ohler,Georg Stoecklin

Nature communications 7:11168 PubMed27041075

2016

Therapeutic activity of modified U1 core spliceosomal particles.

Applications

Unspecified application

Species

Unspecified reactive species

Malgorzata Ewa Rogalska,Mojca Tajnik,Danilo Licastro,Erica Bussani,Luca Camparini,Chiara Mattioli,Franco Pagani
View all publications
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Product promise

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