Rabbit Recombinant Monoclonal U1-C antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | ICC/IF | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected | Expected |
Rat | Expected | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/40 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.
U1 small nuclear ribonucleoprotein C, U1 snRNP C, U1-C, U1C, SNRPC
Rabbit Recombinant Monoclonal U1-C antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR16034
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
U1-C also known as U1 small nuclear ribonucleoprotein C is a component of the U1 snRNP complex. It has a molecular mass of approximately 17 kDa and is expressed in the nucleus of eukaryotic cells. This protein plays an essential role in the spliceosome machinery which is responsible for pre-mRNA splicing. U1-C is part of a highly conserved group of proteins that engage in RNA processing and modification.
U1-C interacts with U1 snRNA and other proteins to form the U1 snRNP an essential component of the spliceosome complex. The spliceosome removes introns from pre-mRNA facilitating the production of mature mRNA. U1-C stabilizes the interaction between the 5' splice site of pre-mRNA and the snRNP playing a significant part in the accuracy of splicing. Its precise function in RNA stability and processing makes it an important aspect of gene expression regulation.
U1-C participates in the splicing pathway ensuring the correct removal of introns during RNA maturation. This protein associates closely with U1 snRNA and other spliceosomal proteins such as U1-70K. It plays a role in the regulation of alternative splicing pathways which can affect gene expression patterns. The U1-C protein indirectly influences processes like cell cycle progression and differentiation through its involvement in RNA splicing.
Malfunction or mutation of U1-C can lead to splicing abnormalities which are linked to diseases such as systemic lupus erythematosus and certain cancers. In lupus autoantibodies target the U1 snRNP complex including U1-C leading to immune system dysfunction. This association highlights the importance of U1-C in maintaining cellular and immune system homeostasis. Additionally aberrant splicing facilitated by altered U1-C function may impact oncogene or tumor suppressor pathways contributing to cancer development.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-U1-C antibody [EPR16034] (ab192028) at 1/5000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: K562 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-U1-C antibody [EPR16034] (ab192028) at 1/10000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: Raw 264.7 cell lysate at 10 µg
Lane 3: PC-12 cell lysate at 10 µg
Lane 4: NIH/3T3 cell lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot analysis of U1-C in K562 lysate immunoprecipitated using ab192028 at 1/40 dilution (Lane 1). Lane 2: PBS instead of K562 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Blocking/dilution buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-U1-C antibody [EPR16034] (ab192028)
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised HeLa cells labeling U1-C with ab192028 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/200 dilution (green). The nuclear counter stain is Dapi (blue).
The two negative controls are ab192028 at 1/500 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
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