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AB155054

Anti-U1A antibody

5

(1 Review)

|

(5 Publications)

Rabbit Polyclonal U1A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNRPA aa 100 to C-terminus.

View Alternative Names

U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-U1A antibody (AB155054)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-U1A antibody (AB155054)

Confocal Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling U1A with ab155054 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling U1A with ab155054 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)

Immunohistochemical analysis of paraffin-embedded rat fore brain tissue stained for SNRPA protein at nucleus with ab155054 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling U1A with ab155054 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)

Immunohistochemical analysis of paraffin-embedded mouse fore brain tissue stained for SNRPA protein at nucleus with ab155054 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U1A antibody (AB155054)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue stained for SNRPA protein at nucleus with ab155054 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western blot - Anti-U1A antibody (AB155054)
  • WB

Supplier Data

Western blot - Anti-U1A antibody (AB155054)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-U1A antibody (ab155054) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SNRPA aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09012

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

U1A also known as snRNP U1A is a protein involved in the splicing of pre-messenger RNA. U1A is approximately 31 kDa in mass and functions as an important component of the U1 small nuclear ribonucleoprotein (snRNP) complex. Expressed in the cell nucleus U1A binds to the stem-loop structures of U1 RNA stabilizing the RNA-protein complex. This binding activity is critical as it allows U1 snRNP to recognize and bind splice sites on precursor mRNA facilitating the splicing process.
Biological function summary

U1A participates in the formation and function of the spliceosome a large RNA-protein complex essential for pre-mRNA processing. U1A as part of the U1 snRNP helps in recognizing and positioning the 5' splice site of introns in pre-mRNA. Through this role U1A ensures proper removal of non-coding sequences allowing for the maturation of mRNA. The spliceosome which includes other components like U2 U4 U5 and U6 snRNPs coordinates finely to execute the precise cutting and ligation of RNA sequences during splicing.

Pathways

U1A plays a central role in the mRNA splicing pathway which is a critical step in gene expression regulation. The splicing pathway interfaces with other biological pathways such as those involved in mRNA translation and degradation. U1A interacts with several proteins within these pathways notably SR proteins and hnRNPs which influence splice site selection and enhance the efficiency of splicing. These interactions highlight the interconnectedness of RNA processing events with overall gene expression controls.

U1A has been implicated in autoimmune diseases such as systemic lupus erythematosus (SLE). In SLE the body generates autoantibodies against several snRNP components including U1A which disrupts normal splicesome function. This leads to defective RNA processing and contributes to disease pathology. Additionally mutations or dysregulation of splicing proteins like SF3B1 and SRSF2 also associated with U1A through splicing abnormalities have been linked to various cancers where they lead to aberrant splicing patterns that drive tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
See full target information SNRPA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:893107 PubMed35860579

2022

Elevated Small Nuclear Ribonucleoprotein Polypeptide an Expression Correlated With Poor Prognosis and Immune Infiltrates in Patients With Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Youfu Zhang,Xuyang Wang,Huaxiang Wang,Yi Jiang,Zhidan Xu,Laibang Luo

Cells 9: PubMed32854341

2020

The Sub-Nuclear Localization of RNA-Binding Proteins in KSHV-Infected Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ella Alkalay,Chen Gam Ze Letova Refael,Irit Shoval,Noa Kinor,Ronit Sarid,Yaron Shav-Tal

Molecular cell 72:369-379.e4 PubMed30340024

2018

RNA Polymerase II Phosphorylated on CTD Serine 5 Interacts with the Spliceosome during Co-transcriptional Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Nojima,Kenny Rebelo,Tomás Gomes,Ana Rita Grosso,Nicholas J Proudfoot,Maria Carmo-Fonseca

RNA (New York, N.Y.) 24:1856-1870 PubMed30254136

2018

Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective -deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Doggett,Ben B Williams,Sebastian Markmiller,Fan-Suo Geng,Janine Coates,Stephen Mieruszynski,Matthias Ernst,Tim Thomas,Joan K Heath

PLoS genetics 13:e1006633 PubMed28234895

2017

A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B.

Applications

ChIP

Species

Mouse

Tatiana A Soboleva,Brian J Parker,Maxim Nekrasov,Gene Hart-Smith,Ying Jin Tay,Wei-Quan Tng,Marc Wilkins,Daniel Ryan,David J Tremethick
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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