Rabbit Polyclonal U2AF1L4 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human U2AF1L4 aa 1 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human U2AF1L4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WU68
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
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Target data
Function
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site (By similarity). Shows a preference for AGC or AGA (By similarity).
Alternative names
Splicing factor U2AF 26 kDa subunit, U2 auxiliary factor 26, U2 small nuclear RNA auxiliary factor 1-like protein 4, U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like 4, U2 small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like protein 3, U2AF1L4, U2AF1L3, U2AF1-RS3
Recommended products
Rabbit Polyclonal U2AF1L4 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human U2AF1L4 aa 1 to C-terminus.
Alternative names
Splicing factor U2AF 26 kDa subunit, U2 auxiliary factor 26, U2 small nuclear RNA auxiliary factor 1-like protein 4, U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like 4, U2 small nuclear RNA auxiliary factor 1-like protein 3, U2AF1-like protein 3, U2AF1L4, U2AF1L3, U2AF1-RS3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human U2AF1L4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WU68
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
U2AF1L4 also known as U2 small nuclear RNA auxiliary factor 1-like 4 is a protein with a mass of about 30 kilodaltons. It plays a role in RNA splicing acting at the early stages of spliceosome assembly. U2AF1L4 binds to the polypyrimidine tract of pre-mRNA introns facilitating the recruitment of U2 snRNP to the branch point sequence. Expression of U2AF1L4 occurs mainly in tissues that are rich in nucleic acids and RNA such as testis and certain types of cancer cells.
- Biological function summary
U2AF1L4 supports the regulation of gene expression through its role in the spliceosome machinery. It may form complexes with other splicing factors such as U2AF2 that are part of the early stages of spliceosomal assembly. This action supports the precise removal of introns and positions the exons accurately for translation. The ability to differentiate splice sites ensures diversity in mRNA transcription which is essential for proper function of genes in diverse tissues.
- Pathways
U2AF1L4 fits into the pre-mRNA splicing pathway. It operates closely with other splicing-associated proteins like SF1 to help in the selection and cleavage of splice sites. Additionally U2AF1L4 interacts within the spliceosome pathway contributing to the splicing outcomes that regulate gene expression and are necessary for maintaining normal cell growth and function.
- Associated diseases and disorders
Irregularities in U2AF1L4 have been linked to certain cancers. Aberrant splicing by U2AF1L4 might play a role in tumor progression where mutations in its coding genes could lead to mis-splicing events. It engages with other proteins implicated in cancer such as the splicing regulator SF3B1 suggesting a complex interplay in tumorigenesis. Understanding these relationships could help develop targeted therapies for related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF1L4 antibody (ab229991)
Paraffin-embedded human breast cancer tissue stained for U2AF1L4 using ab229991 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF1L4 antibody (ab229991)
Paraffin-embedded human testis tissue stained for U2AF1L4 using ab229991 at 1/100 dilution in immunohistochemical analysis.
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